Anti-Visinin-like protein 1 (VLP-1) Antibody (2D11)
Our Anti-Visinin-like protein 1 (VLP-1) mouse monoclonal primary antibody detects bovine, human, mouse, other mammals (predicted), and rat Visinin-like protein 1 (VLP-1), and is IgG. It is validated for use in ICC, IHC-Frozen, WB.
Western blot of bovine cerebellum homogenate stained with M-1657-100.
Visinin (sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1) was originally isolated biochemically from chicken retina as a major protein of about 24 kDa on SDS-PAGE (1). Following cloning and sequencing of visinin, several visinin like proteins were discovered by homology screening (2, 3). One of these, Visinin-like protein 1 is a small Calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression (4, 5). It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein belongs to the large superfamLy of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the confomation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding. Visinin-like protein 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of 22 kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury (6, 7, 8).
IgG
Monoclonal
2D11
IgG1
ICC, IHC, WB
Mouse
22 kDa
Recombinant Visinin-like protein 1 expressed and purified from E. coli.
Human
Bovine, Human, Mouse, Rat
Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Lyophilized
Protein G purified
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
![Left: VILIP-1 staining of rat cerebellum section by Immunohistochemistry. Section was stained with mouse antibody to VILIP-1 (red, 1:500) and rabbit antibody to GFAP (R-1374-50, green, 1:5,000). Blue: DAPI nuclear stain.IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The VILIP-1 antibody reveals protein expressed in granule cell membranes and in synapses in the white matter and molecular layers of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astroglia. Right: Western blot analysis of VILIP-1 expression (green, 1:1,000) in tissue lysates. [1] protein standard, [2] rat brain [3] rat cerebellum, and [4] mouse brain. The band at around 20 kDa corresponds to VILIP-1 protein.](http://www.antibodiesinc.com/cdn/shop/files/m-1657-100-ihc-wb_120x120.jpg?v=1759273347)
