Anti-Vimentin Protein Antibody

Name: Anti-Vimentin Protein Antibody
Brand: Aves Labs
SKU: VIM
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet

Three different antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_035831) and human (NP_003371) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Affinity-Purified IgY

Gene Name

VIM

Molecular Weight

52 kDa

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Immunostaining of adult mouse cerebellum, showing staining in the granule cell layer and white matter tracts. Anti-vimentin antibody, 1:1000; HRP-labeled goat anti-chicken IgY, 1:500.

Culture of neurosphere cells from an e16 mouse brain immunostained for vimentin immunoreactivity. Vimentin antibody, 1:1000. Secondary, fluorescein-labeled goat anti-chicken antibody (Aves Labs, Cat.No. F-1005), 1:500. Hoda Ilias, Aves Labs.

Vimentin western blot of a homogenate prepared from a neurosphere culture. Vimentin antibody, 1:2000; Secondary, HRP-labeled goat anti-chicken antibody (Aves Labs, Cat.No. H-1004), 1:1000. Hoda Ilias, Aves Labs.

Immunostaining of adult mouse cerebellum, showing staining in the granule cell layer and white matter tracts. Anti-vimentin antibody, 1:1000; HRP-labeled goat anti-chicken IgY, 1:500.

Volume: 1000 µL

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Product Details

Target

Vimentin

Target Description

Human Vimentin is a 53,521 dalton protein (466 amino acids) expressed in many cell types of mesodermal origin and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, Vimentin is expressed by migrating neural crest cells forming the PNS and by neural stem cells of the CNS. In some forms of glioblastoma, Vimenin is re-expressed by the tumor cells and serves as a general indicator of their undifferentiated state.

Format

Affinity-Purified IgY

Concentration

300 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

VIM

Molecular Weight

52 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313565

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the vimentin gene product, but are shared between the human (NP_003371, NCBI) and mouse (NP_035831, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS,” pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:2000-1:5000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Vimentin

UniProt Details

UniProt (Immunogen Species): P08670

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Anti-Vimentin Protein Antibody

Three different antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_035831) and human (NP_003371) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.