Anti-Vimentin Antibody (LN6)

Name: Anti-Vimentin Antibody
Brand: PhosphoSolutions
SKU: 2106-VIM
Price: 456.0 USD
Availability: InStock

Our Anti-Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects Vimentin and is Protein L purified. It is great for use in WB, IHC.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.

Click on image to zoom

Species Reactivity

Bovine, Canine, Human, Mouse, Pig, Rabbit, Rat

Applications

ELISA, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 2106-VIM

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Vimentin

Scientific Background

Vimentin is the major protein subunit of the 10 nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).

Format

Protein L Purified

Clonality

Monoclonal

Clone

LN6

Isotype

IgM

Applications

ELISA, IHC, WB

Host Species

Mouse

Gene Name

VIM

Molecular Weight

50 kDa

Immunogen

Cell nuclei preparation from adult human thymus.

Immunogen Species

Human

Species Reactivity

Bovine, Canine, Human, Mouse, Pig, Rabbit, Rat

Antibody Registry ID

AB_2890156

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Protein L purified culture supernatant

Buffer

100 µl in PBS

Dilution Ranges

WB: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

Specific for the ~50 kDa Vimentin protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; Vimentin

UniProt ID

P08670
UniProt Summary: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity). {ECO:0000250|UniProtKB:A0A8C0N8E3, ECO:0000250|UniProtKB:P20152, ECO:0000250|UniProtKB:P31000}.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. {ECO:0000269|PubMed:21746880}.

NCBI Gene ID

7431

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Blue Ice

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Anti-Vimentin Antibody (LN6)

SKU: 2106-VIM

Product Details

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