Anti-Vimentin Antibody

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Our Anti-Vimentin primary antibody from PhosphoSolutions is chicken polyclonal. It detects human, mouse, and rat Vimentin and is total IgY fraction. It is great for use in WB, IHC, ICC.

Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, cat. 2105-VIM, green; Anti-E-cadherin, red). Photo courtesy of Rolf Brekken, University of Texas Southwestern. CC-BY-4.0
Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~50 kDa Vimentin protein.
Immunostaining of HeLa cells labelled with anti-vimentin antibody (cat.2105-VIM, 1:10,000, green), anti-actin (red), and DAPI (blue) labelling of nuclear DNA. The vimentin antibody labels the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Immunostaining of mixed neuron/glial cultures stained with anti-Vimentin antibody (cat. 2105-VIM, 1:500, green) and anti-GFAP antibody (cat. 620-GFAP, 1:1000, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-vimentin antibody (cat. 2105-VIM, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin (cat. 1630-PER, red, 1:500) and anti-vimentin (cat. 2105-VIM, green, 1:10,000). The blue is Hoechst staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.
Immunolabeling of HeLa cells stained with anti-nuclear pore complex antibody (cat. 1515-NPC, red, 1:100) and rabbit anti-vimentin antibody (cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA. The anti-nuclear pore complex antibody reveals strong granular labeling of the nuclei corresponding to the protein. The anti-vimentin antibody specifically labels intermediate filaments.
Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody (cat. 1515-NPC, green, 1:100) and rabbit anti-vimentin antibody (cat. 2105-VIM, red, 1:500).
Immunoflourescence of HeLa cells stained with Anti-Park7 (cat. 1597-PARK, green, 1:1000) showing strong cytoplasmic staining and Anti-Vimentin (cat. 2105-VIM, red, 1:10,000) labeling cytoplasmic intermediate filaments. The blue is nuclear staining.
Immunoflourescence of HeLa cells labeled with anti-SAP49 (cat. 1900-SAP, red, 1:1000) and anti-vimentin (cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.
Immunofluorescence of HeLa cells showing strong granular nuclear staining of SAP49 (red, 1:1000) and vimentin (cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.
Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, cat. 2105-VIM, green; Anti-E-cadherin, red). Photo courtesy of Rolf Brekken, University of Texas Southwestern. CC-BY-4.0
Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, cat. 2105-VIM, green; Anti-E-cadherin, red). Photo courtesy of Rolf Brekken, University of Texas Southwestern. CC-BY-4.0
Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~50 kDa Vimentin protein.
Immunostaining of HeLa cells labelled with anti-vimentin antibody (cat.2105-VIM, 1:10,000, green), anti-actin (red), and DAPI (blue) labelling of nuclear DNA. The vimentin antibody labels the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Immunostaining of mixed neuron/glial cultures stained with anti-Vimentin antibody (cat. 2105-VIM, 1:500, green) and anti-GFAP antibody (cat. 620-GFAP, 1:1000, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-vimentin antibody (cat. 2105-VIM, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin (cat. 1630-PER, red, 1:500) and anti-vimentin (cat. 2105-VIM, green, 1:10,000). The blue is Hoechst staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.
Immunolabeling of HeLa cells stained with anti-nuclear pore complex antibody (cat. 1515-NPC, red, 1:100) and rabbit anti-vimentin antibody (cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA. The anti-nuclear pore complex antibody reveals strong granular labeling of the nuclei corresponding to the protein. The anti-vimentin antibody specifically labels intermediate filaments.
Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody (cat. 1515-NPC, green, 1:100) and rabbit anti-vimentin antibody (cat. 2105-VIM, red, 1:500).
Immunoflourescence of HeLa cells stained with Anti-Park7 (cat. 1597-PARK, green, 1:1000) showing strong cytoplasmic staining and Anti-Vimentin (cat. 2105-VIM, red, 1:10,000) labeling cytoplasmic intermediate filaments. The blue is nuclear staining.
Immunoflourescence of HeLa cells labeled with anti-SAP49 (cat. 1900-SAP, red, 1:1000) and anti-vimentin (cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.
Immunofluorescence of HeLa cells showing strong granular nuclear staining of SAP49 (red, 1:1000) and vimentin (cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.

Bovine, Human, Mouse, Rat
ICC, IHC, WB
Chicken

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SKU: 2105-VIM

Volume: 100 µL
Price:
$375.00

Product Specific References for Applications and Species

  • Immunohistochemistry: Mouse
Immunohistochemistry: Mouse
PMID Dilution Publication
26206560 not listed Kirane, A., et al. 2015. Warfarin blocks Gas6-mediated Axl activation required for pancreatic cancer epithelial plasticity and metastasis. Cancer Research, 75(18), pp.3699-3705.
25060520 not listed Ostapoff, K. T., et al. 2014. Neutralizing murine TGFβR2 promotes a differentiated tumor cell phenotype and inhibits pancreatic cancer metastasis. Cancer Research, 74(18), 4996-5007.
23696215 not listed Ostapoff, K. T., et al. 2013. PG545, an angiogenesis and heparanase inhibitor, reduces primary tumor growth and metastasis in experimental pancreatic cancer. Molecular Cancer Therapeutics, 12(7), pp.1190-1201.
22829202 not listed Kirane, A., et al. 2012. Apricoxib, a novel inhibitor of COX-2, markedly improves standard therapy response in molecularly defined models of pancreatic cancer. Clinical Cancer Research, 18(18), pp.5031-5042
22678114 not listed Kirane, A., et al. 2012. Epithelial-mesenchymal transition increases tumor sensitivity to COX-2 inhibition by apricoxib. Carcinogenesis, 33(9), 1639-46.

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