Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) Antibody

Name: Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) Antibody
Brand: PhosphoSolutions
SKU: 2250-VMAT2C
Price: 456.0 USD
Availability: InStock

Our Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Vesicular Monoamine Transporter 2, C-terminus (VMAT2) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat striatal lysate showing specific immunolabeling of the ~57 kDa VMAT2 protein.

Immunostaining against ChAT (red) and VMAT2 (Cat. 2250-VMAT2C, 1:200, green) in retinal sections of control (e) and Parkinson's Desease (f) patients. Image from publication CC-BY-4.0. PMID: 37013599

Retinal sections immunostained against TH (red), ChAT (green) and vesicular monoamine transporter 2 (VMAT2, blue). S1/S2: stratum 1 and 2 in the IPL. S3/S4: stratum 3 and 4 in the IPL. Scale bar: 20 μm. Image from publication CC-BY-4.0. PMID: 37013599

Western blot of rat striatal lysate showing specific immunolabeling of the ~57 kDa VMAT2 protein.

Click on image to zoom

Species Reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host Species

Sheep

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Data Sheet

Trial Size

Pooled Serum

SKU: 2250-VMAT2C

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Vesicular Monoamine Transporter 2, C-terminus (VMAT2)

Scientific Background

Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996). The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Host Species

Sheep

Gene Name

VMAT2

Molecular Weight

57 kDa

Immunogen

Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region of human VMAT2, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Human

Species Reactivity

Human, Rat

Antibody Registry ID

AB_2492286

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled sheep serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~57 kDa VMAT2 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

1110037L13Rik; 9330105E13; vesicular monoamine member 2; MGC120477; MGC120478; MGC26538; MGC90556; MNAT; Monoamine neurotransmitter transporter; Monoamine transporter; OTTHUMP00000020576; SLC18A2; Solute carrier family 18 (vesicular monoamine member 2; Solute carrier family 18 member 2; SVAT; SVMT; Synaptic vesicle amine transporter brain; Synaptic vesicle monoamine transporter brain; Synaptic vesicular amine transporter; VAT 2; VAT2; Vesicle monoamine transporter type 2; Vesicle monoamine/H+ antiporter; Vesicular amine transporter 2; Vesicular monoamine transporter 2; VMAT 2; VMAT2; VMAT2_HUMAN; Solute carrier family 18

UniProt ID

Q05940
UniProt Summary: Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin (PubMed:23363473, PubMed:37914936, PubMed:38081299, PubMed:38517752, PubMed:8643547). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals (By similarity). Acts as a primary transporter for striatal dopamine loading ensuring impulse-dependent release of dopamine at the synaptic cleft (By similarity). Responsible for histamine and serotonin storage and subsequent corelease from mast cell granules (PubMed:8860238). {ECO:0000250|UniProtKB:Q01827, ECO:0000250|UniProtKB:Q8BRU6, ECO:0000269|PubMed:23363473, ECO:0000269|PubMed:37914936, ECO:0000269|PubMed:38081299, ECO:0000269|PubMed:38517752, ECO:0000269|PubMed:8643547, ECO:0000269|PubMed:8860238}.

NCBI Gene ID

6571

Shipping

Blue Ice

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Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) Antibody

SKU: 2250-VMAT2C

Product Details

Bulk Order Anti-Vesicular Monoamine Transporter 2, C-terminus (VMAT2) Antibody

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