Anti-VASP (C-terminal region) Antibody (M277)
Our VASP (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human VASP (C-terminal region) and is Protein A purified. It is great for use in WB, ICC.
SKU: VM2771
Product Details
VASP (C-terminal region)
Actin filament tethering and bundling are important mechanisms involved in actin superstructure assembly. The ENA/VASP family includes VASP, mena, and Ena-Vasp-like (EVL). These multidomain proteins localize to the leading edge of filopodia where they associate with AFs, interact with profilin, and compete with capping proteins at the barbed end of AFs. Artificial relocalization of VASP from the plasma membrane to mitochondrial membranes inhibits filopodial formation and axon branching, while deletion of all three ENA/VASP proteins produces defects in cortical axon-tract formation. Regulation of VASP protein activity occurs through phosphorylation at Ser-157, Ser-239, and Thr-278. AMPK phosphorylates Thr-278, leading to impaired actin stress fiber assembly and changes in cell morphology.
Protein A Purified
Monoclonal
M277
IgG1
ICC, WB
Mouse
VASP
46/50
Clone (M277) was generated from a recombinant protein that includes amino acids from the C-terminal region of human VASP.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:50
ICC: 1:50
Unconjugated
This antibody detects 46 and 50 kDa* proteins corresponding to the apparent molecular mass of VASP on SDS-PAGE immunoblots of human A431, HeLa, and HUVEC.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
vasodilator-stimulated phosphoprotein
UniProt (Human): P50552
UniProt (Immunogen Species): P50552
UniProt (Immunogen Species): P50552