Anti-VAPA/B Antibody (N479/107)

Name: Anti-VAPA/B Antibody
Brand: NeuroMab
SKU: 75-496
Price: 329.0 USD
Availability: InStock

Our Anti-VAPA/B mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N479/107. It detects mouse and rat VAPA/B, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Representative images of transfected neurons (white arrow) expressing GCaMP or MitoPAGFP (green) and VAP CRISPR-Cas9 sgRNAs (e) and immunostained for VAP (cat. 75-496, 1:100; white) show reduced VAP immunofluorescence compared to untransfected, Control neurons (white arrowheads), identified using Map2. Similar results were obtained with VAP shRNA KD. Image from publication CC-BY-4.0. PMID:38177103

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged VAPA, VAPB or untagged Kv2.1 plasmid probed with N479/107 (left) or N86/8 (right) TC supe..

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Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-496

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VAPA/B

Scientific Background

VAPA and VAPB are members of the VAMP-associated protein family (VAP) (aka vesicle-associated membrane proteins) which are abundant scaffolding proteins predominantly localized to the endoplasmic reticulum (ER). VAPs have recently been shown to interact with Kv2.1 and Kv2.2 channels forming junctions to link the ER to the plasma membrane (Johnson B. et al., 2018).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N479/107

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Vapa Vap33

Molecular Weight

30 kDa

Immunogen

Fusion protein amino acids 1-219 (N-terminus) of rat VAPA (accession number Q9Z270) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2722711

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

IHC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

VAPA (Vesicle-associated membrane protein-associated protein A) (VAMP-associated protein A) (33 kDa VAMPassociatedprotein) (VAMP-A) (VAP-33)

UniProt ID

Q9Z270

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
38177103	1:100	Bapat, O., et al. 2024. VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity. Nature Communications, 205.

Immunohistochemistry: Rat
PMID	Dilution	Publication
33449375	1:250	Deardoff, A.S., et al. 2021. Location, location, location: the organization and roles of potassium channels in mammalian motoneurons. Journal of Physiology, 1391-1420.

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Anti-VAPA/B Antibody (N479/107)

SKU: 75-496

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-VAPA/B Antibody

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