Mouse auditory neurons expressing a V5-tagged reporter protein were stained with Aves Labs chicken anti-V5 antibody at 1:500. Photo courtesy of Thomas Coate.
Bulk Order Anti-V5 Antibody
The V5 epitope tag is derived from a protein epitope found in the P and V proteins of paramyxovirus from the simian virus 5 family. The V5 tag is generally added as an epitope tag to proteins using all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence. Proteins tagged with the V5 epitope tag are detected with high sensitivity and specificity using our chicken anti-V5 antibody using various applications including western blot, immunocytochemistry, and immunohistochemistry.
ICC, IHC, WB
Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on a V5 overexpression cell lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.