Anti-V5 Antibody

Name: Anti-V5 Antibody
Brand: Aves Labs
SKU: ET-V5-0100
Price: 410.0 USD
Availability: InStock

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Our anti-V5 chicken primary antibody from AvesLabs is produced in-house. It detects the 14 amino acid V5 epitope tag, and is purified. It is provided in liquid form.

Clonality

Polyclonal

Applications

ICC, IHC, WB

Host Species

Chicken

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Western blot of cell lysate from HEK293 cells transfected with V5-tagged cDNA (lane 1) or mock transfected cells (lane 2) using chicken α-V5 antibody showing specific immunolabeling at the expected molecular weight.

Immunofluoresence of COS7 cells expressing a V5-tagged protein and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.

Volume: 500 µL

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Price:

$410.00

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Product Details

Target

Target Description

The V5 epitope tag is derived from a protein epitope found in the P and V proteins of paramyxovirus from the simian virus 5 family. The V5 tag is generally added as an epitope tag to proteins using all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence. Proteins tagged with the V5 epitope tag are detected with high sensitivity and specificity using our chicken anti-V5 antibody using various applications including western blot, immunocytochemistry, and immunohistochemistry.

Format

Affinity purified

Concentration

0.2mg/mL

Clonality

Polyclonal

Applications

ICC, IHC, WB

Host Species

Chicken

Antigen

Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on a V5 overexpression cell lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

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Anti-V5 Antibody

Our anti-V5 chicken primary antibody from AvesLabs is produced in-house. It detects the 14 amino acid V5 epitope tag, and is purified. It is provided in liquid form.

Anti-V5 Antibody
SKU: ET-V5-0100

Product Details

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Anti-V5 Antibody

Our anti-V5 chicken primary antibody from AvesLabs is produced in-house. It detects the 14 amino acid V5 epitope tag, and is purified. It is provided in liquid form.

Anti-V5 Antibody SKU: ET-V5-0100

Product Details

Anti-V5 Antibody
SKU: ET-V5-0100