Anti-UFSP2 Antibody

Name: Anti-UFSP2 Antibody
Brand: Aves Labs
SKU: UFSP2-0100
Price: 481.0 USD
Availability: InStock

Our Anti-UFSP2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It is KO validated, detects human, mouse, and rat UFSP2, and is affinity-purified IgY. Works in WB, ICC, IHC.

Immunofluorescent staining for detection of UFSP2 in HeLa cells using Aves Labs chicken anti-UFSP2 polyclonal antibody (Cat No. UFSP2-0100). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-UFSP2 antibody (Cat. UFSP2-0100) was incubated with the sample at a dilution of 1:100 (2 ug/mL) at 4C overnight. After washing with PBST, the cells were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei.

Western blot detection of UFSP2 in UFSP2-KO (lane 1) and wild-type (lane 2) HEK293T cell lysates using Aves Labs chicken anti-UFSP2 polyclonal antibody (Cat No. UFSP2-0100). 10-20% Tris-Glycine gel was loaded with 10 ug of WT and UFSP2-KO HEK293T cell lysates. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Anti-UFSP2 antibody was incubated with the membrane at a concentration of 1 ug/ml and then detected via chemiluminescence (30 min) using a Goat Anti-Chicken H+L, HRP conjugated secondary antibody. Anti-UFSP2 recognizes endogenous UFSP2 only in the wild-type cell lysates at the expected molecular weight of ~52 kDa.

Immunofluorescent staining for detection of UFSP2 in HeLa cells using Aves Labs chicken anti-UFSP2 polyclonal antibody (Cat No. UFSP2-0100). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-UFSP2 antibody (Cat. UFSP2-0100) was incubated with the sample at a dilution of 1:100 (2 ug/mL) at 4C overnight. After washing with PBST, the cells were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

KO Validated

Trial Size

SKU: UFSP2-0100

Volume: 500 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

UFSP2

Scientific Background

The UFSP2 protein (Ufm1-specific protease 2) is a critical regulator of 'ufmylation,' a post-translational modification where the ubiquitin-like modifier Ufm1 is attached to target proteins. Identified in the mid-2000s, UFSP2 serves as the primary de-UFMylase, the enzyme responsible for removing Ufm1 from substrates, while working alongside UFSP1 to facilitate Ufm1 maturation. Its clinical relevance is significant: mutations in the UFSP2 gene are the established cause of Beukes familial hip dysplasia (an autosomal dominant skeletal disorder) and have been increasingly linked to recessive forms of early-onset epileptic encephalopathy and developmental delay. By regulating the ufmylation pathway, UFSP2 helps cells manage endoplasmic reticulum (ER) stress and maintain protein homeostasis, making it an important subject in research for treating genetic, neurodevelopmental, and skeletal pathologies.

Format

Affinity-Purified IgY

Concentration

0.2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

UFSP2

Molecular Weight

53 kDa

Immunogen

Recombinant protein encompassing the C-terminal fragment of human UFSP2.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:500-1:1000
IHC: 1:100-1:500
ICC: 1:100-1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

C4orf20, UFSP2, Ufm1-specific protease 2, UfSP2

UniProt ID

Q9NUQ7

Entrez Gene Details

Entrez Gene Summary: This gene encodes a highly conserved cysteine protease. The protein cleaves two C-terminal residues from ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein. Activation of ubiquitin-fold modifier 1 by the encoded protein exposes a C-terminal glycine residue that allows interaction with other proteins and transfer to its target protein. An allelic variant of this gene has been associated with Beukes hip dysplasia. Alternative splicing results in multiple transcript variants.

NCBI Gene ID

55325

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Anti-UFSP2 Antibody

SKU: UFSP2-0100

Product Details

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