Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5) Antibody

Name: Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5) Antibody
Brand: Biosensis
SKU: C-1406-50
Price: 337.0 USD
Availability: InStock

Our Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5) chicken polyclonal primary antibody detects bovine, human, mouse, other mammals (predicted), pig, and rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5), and is IgY preparation. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

Image shows rat mixed neuron/glial cultures stained with Chicken polyclonal antibody to Ubiquitin C Terminal Hydrolase 1 C-1406-50 (green) and Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein R-1374-50 (red). Blue is a DNA stain. Note that the Ubiquitin antibody stains neurons strongly and specifically and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.

Left: Analysis of UCHL1 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry with chicken antibody to UCHL1 (1:500, red). Cells were co-stained with a mouse antibody to vimentin (green). Blue: DAPI nuclear stain. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells. Right: Western blot analysis of tissue and cell lysates using chicken antibody to UCHL1 (1:2,000, green), and a mouse anti-actin antibody (red, lanes 2-7). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties, but not in lysates of HeLa, NIH-3T3 and other non-neuronal cells. Actin is detected with apparent molecular weight of 42 kDa and provides an excellent loading control.

Image shows rat mixed neuron/glial cultures stained with Chicken polyclonal antibody to Ubiquitin C Terminal Hydrolase 1 C-1406-50 (green) and Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein R-1374-50 (red). Blue is a DNA stain. Note that the Ubiquitin antibody stains neurons strongly and specifically and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.

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Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1406-50

Volume/Size: 50 µL

Variant

Price:

$337.00

Quantity:

Ships: 5-7 business days

Product Details

Target

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5)

Scientific Background

This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).

Format

IgY preparation

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Molecular Weight

24 kDa

Immunogen

Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.

Immunogen Species

Human

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized IgY preparation, with sodium azide.

Dilution Ranges

WB: 1:5000-1:10000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, rat, Ms, Bov, Por. Predicted to react with other mammalian tissues due to sequence homology.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Epididymis luminal protein 117; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Epididymis secretory protein Li 53; UCH-L1; HEL 117; Neuron cytoplasmic protein 9.5; HEL S 53; PGP 9.5; NDGOA; PGP9.5; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; OTTHUMP00000218137; UCHL1; OTTHUMP00000218139; Ubiquitin C Terminal Hydrolase 1; OTTHUMP00000218140; OTTHUMP00000218141; Park 5; PARK5; PGP 9.5; PGP9.5; PGP95; Protein gene product 9.5; Ubiquitin C terminal esterase L1; Ubiquitin C terminal hydrolase; Ubiquitin C terminal hydrolase L1; Ubiquitin carboxyl terminal esterase L1; Ubiquitin carboxyl terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; Ubiquitin thiolesterase; Ubiquitin thiolesterase L1; UCH-L1; UCHL1; UCHL1_HUMAN

UniProt ID

P09936
UniProt Summary: Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways. Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production. Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes. Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis. Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators. Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins. Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation.

NCBI Gene ID

7345

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Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5) Antibody

SKU: C-1406-50

Product Details

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