Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) Antibody
Our Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) rabbit polyclonal primary antibody detects bovine, chicken, horse, human, mouse, pig, and rat Ubiquitin C Terminal Hydrolase 1 (UCHL1), and is whole serum. It is validated for use in ICC, WB.
![Left: Analysis of SH-SY5Y cells for UCHL1 expression with rabbit antibody to UCHL1 (green, 1:1,000) by Immunocytochemistry. Cells were co-stained with mouse antibody to fibrillarin (M-1372-250, red, 1:1,000). Blue: DAPI nuclear stain. The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the fibrillarin antibody specifically labels nucleoli. Right: Western blot analysis of tissue and cell lysates using rabbit antibody to UCHL1 (red, 1:2,000, lanes 2-7). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24 kDa corresponds to the UCHL1 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties, but not in HeLa, NIH-3T3 and other non-neuronal cells. The blot was simultaneously probed with a mouse antibody to HSP60 (green).](http://www.antibodiesinc.com/cdn/shop/files/r-1408-50-ihc-wb_120x120.jpg?v=1759274461)
Image shows rat mixed neuron/glial cultures stained with Rabbit polyclonal antibody to Ubiquitin C Terminal Hydrolase 1 R-1408-50 (green). Blue is a DNA stain. Note that the Ubiquitin C Terminal Hydrolase 1 (UCHL1) antibody stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also. Surrounding glial cells are not stained with this antibody, though many are present as visualized using the DNA stain.
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