Anti-Tyrosine Hydroxylase (TH) Antibody

Name: Anti-Tyrosine Hydroxylase (TH) Antibody
Brand: Biosensis
SKU: R-1645-500
Price: 327.0 USD
Availability: InStock

Our Anti-Tyrosine Hydroxylase (TH) rabbit polyclonal primary antibody detects guinea pig, human, mouse, and rat Tyrosine Hydroxylase (TH), and is IgG. It is validated for use in IHC-Frozen.

Immunohistochemical staining of catecholaminergic neurons in the rat brain stem.

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Species Reactivity

Guinea Pig, Human, Mouse, Pig, Rat

Applications

IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1645-500

Volume/Size: 500 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Tyrosine Hydroxylase (TH)

Scientific Background

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.

Format

IgG

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Immunogen

A synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47).

Immunogen Species

Human

Species Reactivity

Guinea Pig, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS, pH 7.4, without preservatives.

Dilution Ranges

WB: 5-15 µg/mL
IHC: 4-10 µg/mL

Application Details

This antiserum stains catecholaminergic neurons, including dendritic processes and fine nerve terminals. We recommend mouse or rat brain containing catecholaminergic neurons as a positive control for this antibody, for example brain stem or striatum.

Conjugate

Unconjugated

Specificity

IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat, mouse and guinea pig. Cross reactivity with other species has not yet been tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

TH; Tyrosine hydroxylase; Tyrosine 3-monooxygenase; L-tyrosine hydroxylase; Tyrosine 3-hydroxylase

UniProt ID

P04177

Shipping

25°C (ambient)

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Anti-Tyrosine Hydroxylase (TH) Antibody

SKU: R-1645-500

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