Anti-Tyrosine Hydroxylase (TH) Antibody
Our Anti-Tyrosine Hydroxylase (TH); Tyrosine 3-monooxyegnase chicken polyclonal primary antibody detects human, mouse, and rat Tyrosine Hydroxylase (TH); Tyrosine 3-monooxyegnase, and is IgY. It is validated for use in FC, ICC, IHC-Frozen, WB.

Left: Visualization of tyrosine hydroxylase expression in rat brain by Immunofluorescent analysis. Section was stained with chicken anti-TH antibody, C-2126-100, (green, 1:5,000) and Hoechst staining of nuclear DNA (blue). Product C-2126-100 stains the dopaminegic TH-positive neurons and their processes of the substantia nigra. Right: Western blot analysis of tissues and lysates using C-2126-100 (green, 1:50,000) with a band at approx. 58kDa in brain tissue lysates. Lane 1: protein standard (red); Lane 2: rat brain caudate/putmen region; Lane 3: rat brain midbrain; Lane 4: whole mouse midbrain; Lane 5: cow midbrain segment.
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SKU: C-2126-100
Product Details
Tyrosine Hydroxylase (TH); Tyrosine 3-monooxyegnase
Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity). (Ref: uniprot.org)
IgY
Polyclonal
IgY
Flow, ICC, IHC, WB
Chicken
The antibody has been made against the full length recombinant human TH (524 amino acid sequence) expressed in and purified from E. Coli
Human
Human, Mouse, Rat
Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
Lyophilized
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
WB: 1:5000-1:10000
IHC: 1:100-1:500
ICC: 1:100-1:500
IHC: 1:100-1:500
ICC: 1:100-1:500
Flow Cytometry at 20 µg/mL
Unconjugated
Human. Species cross-reactivity includes rat and mouse.
For research use only.
United States
12 months after date of receipt (unopened vial).
TH; T-H; tyrosine 3-monooxygenase; monooxygenase; oxidoreductase
25°C (ambient)