Our Anti-Tyrosine Hydroxylase (Ser31) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Tyrosine Hydroxylase (Ser31) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Western blot of PC-12 cell lysate incubated in the absence (-) and presence (+) of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase phosphorylated at Ser31.
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, IHC, WB
Rabbit
TH
60 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Non-Human Primate
AB_2492278
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser31.
Phosphorylated
Ser31
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
Witkovsky, P, et al. 2000. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. Journal of chemical neuroanatomy, 19(2), pp.105-116.
Witkovsky, P, et al. 2000. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. Journal of chemical neuroanatomy, 19(2), pp.105-116.
Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
Salvatore, M.F, et al. 2001. Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of neurochemistry, 79(2), pp.349-360.
Le, VQ, et al. 2023. Extracellular H+ Ions are a Novel Signal for Tyrosine Hydroxylase Activation in Catecholaminergic Cells. ACS Chemical Neuroscience, 1774-1784.
Salvatore, M.F., et al. 2018. Prolonged increase in ser31 tyrosine hydroxylase phosphorylation in substantia nigra following cessation of chronic methamphetamine. Neurotoxicology, 67, pp.121-128.
Nakashima, A., et al. 2020. NT5DC2 affects the phosphorylation of tyrosine hydroxylase regulating its catalytic activity. Journal of Neural Transmission, pp.1-10.
Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
Izumi, Y, et al. 2014. Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death. Toxicological Sciences, 139(2), pp.466-478.
Jedynak, J.P., et al. 2002. Acute administration of cocaine regulates the phosphorylation of serine‐19,‐31 and‐40 in tyrosine hydroxylase. Journal of neurochemistry, 82(2), pp.382-388.
Salvatore, M.F, et al. 2001. Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of neurochemistry, 79(2), pp.349-360.
Bulk Order Anti-Tyrosine Hydroxylase (Ser31) Antibody
