Anti-Tyrosine Hydroxylase Antibody (Purified) (LNC1)

Name: Anti-Tyrosine Hydroxylase Antibody (Purified)
Brand: Antibodies Incorporated
SKU: 77-700
Price: 360.0 USD
Availability: InStock

from Antibodies Incorporated Datasheet Trial Size

98 Citations

Product Type

Primary Antibody

Species Reactivity

Chicken, Frog, Human, Lizard, Monkey, Mouse, Rat, Vole, Zebrafish

Clone

LNC1

Applications

IHC, IP, WB

Host Species

Mouse

Format

Purified

Gene Name

TH, TYH, EC 1.14.16.2

Molecular Weight

59-63 kDa

Go to Full Product Details

Double-labeled fluorescence immunohistochemistry of human midbrain dopamine neurons stained green with LNC1 and red with PitX.

Western Blot of HEK293 cells transfected to express human Tyrosine Hydroxylase isoform 2 and Green Fluorescent Protein demonstrates the specificity of mouse monoclonal antibody LNC1 to tyrosine hydroxylase protein.

LNC1 anti-TH monoclonal antibody was used at 2.5ug/mL to stain endogenous tyrosine hydroxylase in rat brain lysate.

Mouse brain midbrain dopamine neurons in the substantia nigra double-labeled with LNC1 for tyrosine hydroxylase (TH, red and cytoplasmic) and a rabbit antibody to Nuclear Factor Y subunit A (NFYA, green and primarily nuclear).

Double-labeled fluorescence immunohistochemistry of human midbrain dopamine neurons stained green with LNC1 and red with PitX.

Volume: 100 µL

Variant

Price:

$360

Quantity:

Bulk Order Anti-Tyrosine Hydroxylase Antibody (Purified)

Product Details

Target

Tyrosine 3-monooxygenase

Target Description

TH is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine and is responsible for converting L-tyrosine to L-dopa. Synthesis of catecholamines is regulated by the interaction of TH with its’ cofactor, tetrahydrobiopterin (BH4) and the substrates L-tyrosine and molecular oxygen. In humans four TH mRNA splice variants (hTH1-hTH4) have been isolated while subprimate species rely on a single form of TH. It is known that the hTH1-hTH4 variants are identical in their catalytic domain but differ in their N-terminal regulatory domains. Importantly, LNC1 reacts with the catalytic domain of TH and thus with all four isoforms of human TH. The role of TH in the synthesis of catecholamine neurotransmitters suggests a connection between the enzyme and a number of neuropathogenic diseases characterized by irregular catecholamine levels, such as Parkinson's disease, schizophrenia, and dystonia, as well as a variety of cardiovascular diseases.

Format

Purified

Clonality

Monoclonal

Clone

LNC1

Isotype

IgG1

Applications

IHC, IP, WB

Host Species

Mouse

Gene Name

TH, TYH, EC 1.14.16.2

Molecular Weight

59-63 kDa

Antigen

Tyrosine Hydroxylase purified from PC12 cells

Species Reactivity

Chicken, Frog, Human, Lizard, Monkey, Mouse, Rat, Vole, Zebrafish

Physical State

Liquid

Buffer

10mM Tris, 50mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe rat brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

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Anti-Tyrosine Hydroxylase Antibody (Purified) (LNC1)

Anti-Tyrosine Hydroxylase Antibody (Purified)
SKU: 77-700

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Anti-Tyrosine Hydroxylase Antibody (Purified) (LNC1)

Anti-Tyrosine Hydroxylase Antibody (Purified) SKU: 77-700

Bulk Order Anti-Tyrosine Hydroxylase Antibody (Purified)

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Anti-Tyrosine Hydroxylase Antibody (Purified)
SKU: 77-700