Anti-Tyrosine Hydroxylase Antibody
Our Anti-Tyrosine Hydroxylase sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects all mammalian Tyrosine Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Immunostaining of free-floating sections of mouse locus ceruleus (brainstem) labeling tyrosine hydroxylase (Cat. 2027-THSHP, red, 1:2000) The mouse was transcardially perfused with 20 mL of PBS containing 0.1% procaine and 100 U/mL heparin followed by 20 mL of 4% paraformaldehyde (PFA) in PBS. The dissected brains were kept in fixative at 4 °C overnight. The image was kindly provided by Eugene Dimitrov, Department of Physiology and Biophysics, Rosalind Franklin University.
Click on image to zoom
Human, Mouse, Rabbit, Rat
ELISA, ICC, IHC, WB
Sheep
SKU: 2027-THSHP
Ships: 1-2 business days
Product Details
Tyrosine Hydroxylase
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, ICC, IHC, WB
Sheep
TH
60 kDa
SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma.
Rat
Human, Mouse, Rabbit, Rat
Chicken
AB_2492277
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled sheep serum by affinity purification using a column to which the fusion protein immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
ICC: 1:1000
WB Brain: 1:1000
IHC: 1:1000
ICC: 1:1000
Unconjugated
Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
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25085
Blue Ice
- Western Blot Protocol: Download

