Anti-Tyrosine Hydroxylase Antibody

Our Anti-Tyrosine Hydroxylase rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects all mammalian Tyrosine Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Western blot detection of tyrosine hydroxylase in rat caudate fraction (lane 1, high expression) and NIH3T3 (lane 2, low expression/negative control) cell lysates using PhosphoSolutions anti-Tyrosine Hydroxylase rabbit polyclonal antibody (Cat No. 2025-THRAB). 4-12% Bis-Tris gel was loaded with 15 µg of rat caudate fraction and NIH3T3 lysates. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Anti-Tyrosine Hydroxylase rabbit (Cat No. 2025-THRAB) (lanes 1, 2) antibodies were incubated with the membrane at a concentration of 1:1000. The primary antibodies were then detected via chemiluminescence (4 seconds) using a goat anti-Rabbit H+L, HRP conjugated secondary antibody. Anti-TH antibodies recognize tyrosine hydroxylase found in brain lysates at ~60 kDa and an absence in signal in mouse NIH3T3 cell lysates, consistent with low endogenous expression levels.
Immunofluorescence of human iPSC-derived dopaminergic neurons labeled with anti-tyrosine hydroxylase (Cat. 2025-THRAB, green, 1:500). The blue stain is DAPI. Before labeling, cells were fixed with 4% PFA and permeabilized with 0.1 to 0.3 % Triton X. This image is kindly provided by Aurelie de Rus Jacquet, Howard Hughes Medical Institute, Janelia Research Campus.
Immunostaining of E17 rat midbrain mixed neuronal cultures showing TH positive neurons (Cat. 2025-THRAB, green, 1:500) and MAP2 (Cat. 1100-MAP2, red, 1:2000). Before labeling, cells were fixed with 4% PFA in PBS and permeabilized for 1 hour 0.3 % Triton X. Image courtesy of Aurélie de Rus Jacquet, laboratory of Dr. Jean-Christophe Rochet, Purdue University.
Western blot detection of tyrosine hydroxylase in rat caudate fraction (lane 1, high expression) and NIH3T3 (lane 2, low expression/negative control) cell lysates using PhosphoSolutions anti-Tyrosine Hydroxylase rabbit polyclonal antibody (Cat No. 2025-THRAB). 4-12% Bis-Tris gel was loaded with 15 µg of rat caudate fraction and NIH3T3 lysates. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Anti-Tyrosine Hydroxylase rabbit (Cat No. 2025-THRAB) (lanes 1, 2) antibodies were incubated with the membrane at a concentration of 1:1000. The primary antibodies were then detected via chemiluminescence (4 seconds) using a goat anti-Rabbit H+L, HRP conjugated secondary antibody. Anti-TH antibodies recognize tyrosine hydroxylase found in brain lysates at ~60 kDa and an absence in signal in mouse NIH3T3 cell lysates, consistent with low endogenous expression levels.
Western blot detection of tyrosine hydroxylase in rat caudate fraction (lane 1, high expression) and NIH3T3 (lane 2, low expression/negative control) cell lysates using PhosphoSolutions anti-Tyrosine Hydroxylase rabbit polyclonal antibody (Cat No. 2025-THRAB). 4-12% Bis-Tris gel was loaded with 15 µg of rat caudate fraction and NIH3T3 lysates. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Anti-Tyrosine Hydroxylase rabbit (Cat No. 2025-THRAB) (lanes 1, 2) antibodies were incubated with the membrane at a concentration of 1:1000. The primary antibodies were then detected via chemiluminescence (4 seconds) using a goat anti-Rabbit H+L, HRP conjugated secondary antibody. Anti-TH antibodies recognize tyrosine hydroxylase found in brain lysates at ~60 kDa and an absence in signal in mouse NIH3T3 cell lysates, consistent with low endogenous expression levels.
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Human, Mouse, Rat
ELISA, ICC, IHC, WB
Rabbit

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Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: 2025-THRAB

Volume: 100 µL
Price:
$456.00
Ships: 1-2 business days

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