Name: Anti-Tyrosine Hydroxylase Antibody
Brand: PhosphoSolutions
SKU: 2025-THRAB
Price: 415.0 USD
Availability: InStock

Our Anti-Tyrosine Hydroxylase rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects all mammalian Tyrosine Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Chicken

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

TH

Molecular Weight

60 kDa

Go to Full Product Details

Immunofluorescence of human iPSC-derived dopaminergic neurons labeled with anti-tyrosine hydroxylase (cat. 2025-THRAB, green, 1:500). The blue stain is DAPI. Before labeling, cells were fixed with 4% PFA and permeabilized with 0.1 to 0.3 % Triton X. This image is kindly provided by Aurelie de Rus Jacquet, Howard Hughes Medical Institute, Janelia Research Campus.

Immunostaining of E17 rat midbrain mixed neuronal cultures showing TH positive neurons (cat. 2025-THRAB, green, 1:500) and MAP2 (cat. 1100-MAP2, red, 1:2000). Before labeling, cells were fixed with 4% PFA in PBS and permeabilized for 1 hour 0.3 % Triton X. Image courtesy of Aurélie de Rus Jacquet, laboratory of Dr. Jean-Christophe Rochet, Purdue University.

Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.

Volume: 100 µL

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Price:

$415

Quantity:

Bulk Order Anti-Tyrosine Hydroxylase Antibody

Product Details

Target

Tyrosine Hydroxylase

Target Description

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

TH

Molecular Weight

60 kDa

Antigen

SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma.

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Chicken

Antibody Registry ID

AB_2492276

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Dystonia 14 antibody, DYT14 antibody, DYT5b antibody, EC 1.14.16.2 antibody, OTTHUMP00000011225 antibody, OTTHUMP00000011226 antibody, ple antibody, Protein Pale antibody, TH antibody, The antibody, TY3H_HUMAN antibody, TYH antibody, Tyrosine 3 hydroxylase antibody, Tyrosine 3 monooxygenase antibody, Tyrosine 3-hydroxylase antibody, Tyrosine 3-monooxygenase antibody, Tyrosine hydroxylase antibody

UniProt Details

UniProt (Human): P07101
UniProt (Immunogen Species): P04177

NCBI Gene ID

25085

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Human | Mouse | Non-Human Primate
Western Blotting: Human | Mouse | Non-Human Primate

Immunocytochemistry: Human
PMID	Dilution	Publication
37515763	1:1000	Sheta, R, et al. 2023. Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons. STAR protocols, 102486.

Immunocytochemistry: Rat
PMID	Dilution	Publication
3354843	1:5000	Jaumotte, J.D., et al. 2021. Protection of dopamine neurons by CDNF and neurturin variant N4 against MPP+ in dissociated cultures from rat mesencephalon. Plos One, 16(2), p.e0245663.
25952507	not listed	Cooper, R., et al. 2015. Silver nanoparticles at sublethal concentrations disrupt cytoskeleton and neurite dynamics in cultured adult neural stem cells. Neurotoxicology, 48, 231-238.

Immunohistochemistry: Human
PMID	Dilution	Publication
33113371	1:200	Ye, Y., et al. 2020. . Endocannabinoid Receptor-1 and Sympathetic Nervous System Mediate the Beneficial Metabolic Effects of Gastric Bypass. Cell Reports, 33(4), p.108270.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
33113371	1:400	Ye, Y., et al. 2020. . Endocannabinoid Receptor-1 and Sympathetic Nervous System Mediate the Beneficial Metabolic Effects of Gastric Bypass. Cell Reports, 33(4), p.108270.
26526844	not listed	Ka, M., et al. 2016. Microtubule-Actin Crosslinking Factor 1 Is Required for Dendritic Arborization and Axon Outgrowth in the Developing Brain. Molecular neurobiology, November, 1-15.

Immunohistochemistry: Non-Human Primate
PMID	Dilution	Publication
33113371	1:400	Ye, Y., et al. 2020. . Endocannabinoid Receptor-1 and Sympathetic Nervous System Mediate the Beneficial Metabolic Effects of Gastric Bypass. Cell Reports, 33(4), p.108270.

Western Blotting: Human
PMID	Dilution	Publication
33113371	1:2000	Ye, Y., et al. 2020. . Endocannabinoid Receptor-1 and Sympathetic Nervous System Mediate the Beneficial Metabolic Effects of Gastric Bypass. Cell Reports, 33(4), p.108270.

Western Blotting: Mouse
PMID	Dilution	Publication
34264434	1:2000	Chu, Y., et al. 2021. Gastric Bypass Sensitizes Sympathetic and Thermogenic Activity of Brown Adipose Tissue to Cold Exposure. Obesity Surgery, 31(10), pp.4653-4656.
33113371	1:2000	Ye, Y., et al. 2020. Endocannabinoid Receptor-1 and Sympathetic Nervous System Mediate the Beneficial Metabolic Effects of Gastric Bypass. Cell Reports, 33(4), p.108270.
26526844	1:8000	Ghersa, F., et al. 2019. Reduced inflammatory state promotes reinnervation of endometriotic-like lesions in TNFRp55 deficient mice. Molecular human reproduction. 2019 May 9. pii: gaz026.

Western Blotting: Non-Human Primate
PMID	Dilution	Publication
33113371	1:2000	Ye, Y., et al. 2020. . Endocannabinoid Receptor-1 and Sympathetic Nervous System Mediate the Beneficial Metabolic Effects of Gastric Bypass. Cell Reports, 33(4), p.108270.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Tyrosine Hydroxylase Antibody

Our Anti-Tyrosine Hydroxylase rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects all mammalian Tyrosine Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.