Anti-Type V Collagen Antibody (1E2-E4/Col5)

Name: Anti-Type V Collagen Antibody
Brand: Antibodies Incorporated
SKU: CSI 006-01-010
Price: 325.0 USD
Availability: InStock

Our mouse Type V Collagen monoclonal antibody from Antibodies Incorporated is produced in-house from clone 1E2-E4/Col5. It detects bovine, canine, human, kangaroo, pig, rabbit, and sheep (ovine) Type V Collagen and is Protein A or Protein G purified. Works in ELISA, IHC, WB.

Anti-Type V Collagen Antibody

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Species Reactivity

Bovine, Canine, Human, Kangaroo

Applications

ELISA, IHC, WB

Host Species

Mouse

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Data Sheet

SKU: CSI 006-01-010

Volume: 100 uL

Variant

Price:

$325.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Type V Collagen

Scientific Background

Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakend connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.

Format

Protein A or Protein G purified

Concentration

1 mg/mL ±15%

Clonality

Monoclonal

Clone

1E2-E4/Col5

Isotype

IgG2a/k

Applications

ELISA, IHC, WB

Host Species

Mouse

Gene Name

COL5A1

Molecular Weight

184 kDa

Immunogen

Acid-digested pepsin soluble dog type V collagen

Immunogen Species

Canine

Species Reactivity

Bovine, Canine, Human, Kangaroo

Storage

2-8°C without exposure to light

Physical State

Liquid

Production Notes

BSA free

Buffer

0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Application Details

CSI 006-01 can be used for detection of collagens by ELISA. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well. In immunoblotting CSI 006-01 detects human type V collagen only in its native triple helix form. CSI 006-01 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.

Conjugate

Unconjugated

Specificity

CSI 006-01 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

COL5A1; Collagen type V alpha 1 chain; EDSC; EDSCL1; FMDMF; collagen alpha-1(V) chain; collagen; type V; alpha 1

UniProt ID

A0A8I3NPQ5
UniProt Summary: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.

NCBI Gene ID

1289

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Anti-Type V Collagen Antibody (1E2-E4/Col5)

SKU: CSI 006-01-010

Product Details

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