Our Anti-Tryptophan Hydroxylase (Ser260) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Tryptophan Hydroxylase (Ser260) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat brainstem lysate showing specific immunolabeling of the ~55 kDa TPH protein phosphorylated at Ser260 in lane one (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (NP), shown in the third lane.
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser-260 which lies within the regulatory domain of TPH (Jiang et al., 2000).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
TPH1
55 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of rat tryptophan hydroxylase (TPH), conjugated to keyhole limpet hemocyanin (KLH).
Human, Rat
Bovine, Canine, Chicken, Mouse, Zebrafish
AB_2492274
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser260. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser260
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Jiang, G.T., Yohrling, G.T., Schmitt, I.V.J.D. and Vrana, K.E., 2000. Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. Journal of Molecular Biology, 302(4), pp.1005-1017. PMID: 10993738
Johansen, P.A., Jennings, I., Cotton, R.G. and Kuhn, D.M., 1996. Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. Journal of Neurochemistry, 66(2), pp.817-823. PMID: 8592157
Martínez, A., Knappskog, P.M. and Haavik, J., 2001. A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Current Medicinal Chemistry, 8(9), pp.1077-1091. PMID: 11472242
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