Our Anti-Tryptophan Hydroxylase sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Tryptophan Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Western blot of human dorsal Raphe nucleus showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein.
Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
IHC, WB
Sheep
TPH1
55 kDa
Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled sheep serum by affinity purification using a column to which the fusion protein immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
IHC: 1:1000
Unconjugated
Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Haycock, JW., et al. 2002. A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope. Journal of Neuroscience Methods, 114(2), pp.205-212.
