Anti-TRPM7 (Extracellular region) Antibody (M573)

Name: Anti-TRPM7 (Extracellular region) Antibody
Brand: PhosphoSolutions
SKU: TM5731
Price: 348.0 USD
Availability: InStock

Our TRPM7 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat TRPM7 (Extracellular region) and is antigen affinity purified. It is great for use in WB.

Western blot image of TRPM7 expression in human MCF-7 cell lysate. The blot was probed with mouse monoclonal anti-TRPM7 (Extracellular region) M573 at a dilution of 1:250.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Mouse

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Data Sheet

SKU: TM5731

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

TRPM7 (Extracellular region)

Scientific Background

The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M573

Isotype

IgG1

Applications

ELISA, WB

Host Species

Mouse

Gene Name

TRPM7

Molecular Weight

220

Immunogen

Clone M573 was generated from TRPM7 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MCF7 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

ALSPDC; TRPM7 TrpC7; CHAK1; LTRPC7; LTrpC-7; TRP-PLIK; LTRPC ion channel family member 7; channel-kinase 1; long transient receptor potential channel 7; TRP

UniProt ID

Q96QT4
UniProt Summary: Bifunctional protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins via its kinase domain. The channel is highly permeable to divalent cations, specifically calcium (Ca2+), magnesium (Mg2+) and zinc (Zn2+) and mediates their influx. Controls a wide range of biological processes such as Ca2(+), Mg(2+) and Zn(2+) homeostasis, vesicular Zn(2+) release channel and intracellular Ca(2+) signaling, embryonic development, immune responses, cell motility, proliferation and differentiation. The C-terminal alpha-kinase domain autophosphorylates cytoplasmic residues of TRPM7. In vivo, TRPM7 phosphorylates SMAD2, suggesting that TRPM7 kinase may play a role in activating SMAD signaling pathways. In vitro, TRPM7 kinase phosphorylates ANXA1 (annexin A1), myosin II isoforms and a variety of proteins with diverse cellular functions.

NCBI Gene ID

54822

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Anti-TRPM7 (Extracellular region) Antibody (M573)

SKU: TM5731

Product Details

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