Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/3 (left) and N86/8 (right) TC supe.
Anti-TRIP8b (Exon 4) Antibody
TRIP8b (exon 4)
PEX5-related protein, Peroxisomal Biogenesis Factor 5 Like, TPR-containing Rab8b-interacting protein or TRIP8b is encoded by the gene Pex5l. It is a member of the peroxisomal targeting signal receptor family. TRIP8b is a cytoplasmic accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression to the dendrites in many types of neurons. TRIP8b plays a role in regulation of HCN channel cyclic nucleotide kinetics. TRIP8b is expressed in the brain. Diseases associated with PEX5L include Rhizomelic Chondrodysplasia Punctata, Type 5 and Rhizomelic Chondrodysplasia Punctata, Type 2.
Pex5l Pex2 Pex5r Trip8b
Fusion protein amino acids 1-192 (exon 1a, exon 4 and constant region) of rat TRIP8b (also known as TPR-containing Rab8b-interacting protein, Peroxisome biogenesis factor 5-like, Peroxin-5 or PEX5-related/like protein, Pex5Rp, Pex5l, Pex5r, Pex2, Pxr2, accession number Q925N3); Mouse: 97% identity (147/151 amino acids identical); Human: 83% identity (180/216 amino acids identical)
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Specific for exon 4(does not react with TRIP8b isoforms containing exons 1a, 1b or 2)
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.