Anti-TRIP8B (Exon 1A/5 Junction) Antibody (N291C/22)

Name: Anti-TRIP8B (Exon 1A/5 Junction) Antibody
Brand: NeuroMab
SKU: 75-334
Price: 329.0 USD
Availability: InStock

1 Citation

Our Anti-TRIP8b (exon 1a/5 junction) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N291C/22. It is KO validated, detects mouse and rat TRIP8b (exon 1a/5 junction), and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunoblot: extracts of COS cells transiently transfected with carboxy- terminally (C) and amino-terminally (N) GFP- tagged TRIP8b-1a-5, TRIP8b-1a-4 or untagged Kv2.1 plasmid and probed with N291C/22 (left) and N212/7 (right) TC supe.

Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N291C/22 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Dane Chetkovich (Northwestern University).

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Species Reactivity

Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-334

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

TRIP8b (exon 1a/5 junction)

Target Description

PEX5-related protein, Peroxisomal Biogenesis Factor 5 Like, TPR-containing Rab8b-interacting protein or TRIP8b is encoded by the gene Pex5l. It is a member of the peroxisomal targeting signal receptor family. TRIP8b is a cytoplasmic accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression to the dendrites in many types of neurons. TRIP8b plays a role in regulation of HCN channel cyclic nucleotide kinetics. TRIP8b is expressed in the brain. Diseases associated with PEX5L include Rhizomelic Chondrodysplasia Punctata, Type 5 and Rhizomelic Chondrodysplasia Punctata, Type 2.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N291C/22

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Pex5l Pex2 Pex5r Pxr2

Molecular Weight

60 kDa

Antigen

Synthetic peptide amino acids 1-12 (MYQGHMQLVNEQ, exon 1a (yellow) and exon 5 (green) of constant region) of mouse TRIP8b (accession number Q8C437)

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315950

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Specific for exon 1a/5 junction (does not react

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

PEX5-related protein (PEX2-related protein) (PEX5-like protein) (Peroxin-5-related protein) (Tetratricopeptide repeat-containing Rab8b-interacting protein) (Pex5Rp) (TRIP8b)

UniProt ID

Q8C437

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
32467357	1:1000	Brandalise, F., et al. 2020. Fragile X Mental Retardation Protein Bidirectionally Controls Dendritic Ih in a Cell Type-Specific Manner between Mouse Hippocampus and Prefrontal Cortex. Journal of Neuroscience, 5327-5340.

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Anti-TRIP8B (Exon 1A/5 Junction) Antibody (N291C/22)

SKU: 75-334

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-TRIP8B (Exon 1A/5 Junction) Antibody

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