Our Anti-TREM2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat TREM2, and is affinity purified. It is great for use in ICC, WB.
Immunofluorescence of TREM2-FLAG transfected COS-7 cells using chicken α-TREM2 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.
Triggering receptor expressed on myeloid cells 2, TREM2, is a type I transmembrane innate immune receptor predominantly expressed by microglia in the central nervous system and is genetically linked to the risk for Alzheimer’s disease (Schmid, C.D., et al., 2002). TREM2 has been shown to be a specific marker for alternatively activated microglia known as M2 cells (Cherry, J.D. et al., 2014). TREM2 binds to its adaptor, DNAX-activating protein (DAP12) on the surface of microglia to enact innate immune responses and downstream cellular responses or signaling pathways (Jay,T.R, et al., 2017). TREM2 has important roles in microglial phagocytosis of apoptotic neurons, damaged myelin, and amyloid plaques (Gratuze, M., et al., 2018). In early brain development, TREM2 is essential for synaptic pruning (Filipello, F. et al., 2018).
Affinity purified
0.2 mg/mL
Polyclonal
IgY
ICC, WB
Chicken
TREM2
36 kDa
Purified recombinant extracellular domain of human TREM2 (Uniprot Q9NZC2) expressed in E.Coli
Human, Mouse, Rat
AB_2910558
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
WB: 1:500-1:1000
ICC: 1:100-1:500
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2
