Our Anti-Thyroid Hormone Receptor, α1/α2-Isotype primary antibody from PhosphoSolutions is mouse monoclonal. It detects human and rat Thyroid Hormone Receptor, α1/α2-Isotype and is Protein G purified. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~50 kDa TR-α1 and the ~58 kDa TR-α2 protein.
Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory (Ambrogini et al., 2005; Chan and Kilby, 2000). Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors TRα1, TRα2, TRβ1 and TRβ2. TRα1, TRβ1 and TRβ2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain (Constantinou et al., 2005).
Protein G Purified
Monoclonal
1718
IgG2a
ELISA, WB
Mouse
THRA
50/58 kDa
Synthetic peptide corresponding to amino acid residues from the N-terminal region of human thyroid hormone receptor, α1/α2-isotype, conjugated to keyhole limpet hemocyanin (KLH).
Human
Human, Rat
Canine, Mouse, Non-Human Primate
AB_2492261
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatent.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~50 kDa TR-α1 and the ~58 kDa TR-α2 proteins.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
c-erbA-1; C-erbA-alpha; EAR-7; ERBA 1; ERBA; ERBA related 7; Nuclear receptor subfamily 1 group A member 1; THA_HUMAN; THRA; Thra1; Thra2; Thyroid hormone receptor alpha 1; Thyroid hormone receptor alpha 2; Thyroid hormone receptor alpha; TR alpha 1; TR alpha 2; Tra1; Triiodothyronine receptor; V-erbA-related protein 7
P10827
UniProt Summary: [Isoform Alpha-1]: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. {ECO:0000269|PubMed:12699376, ECO:0000269|PubMed:14673100, ECO:0000269|PubMed:18237438, ECO:0000269|PubMed:19926848}.; [Isoform Alpha-2]: Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action. {ECO:0000269|PubMed:8910441}.
Ambrogini, P., Cuppini, R., Ferri, P., Mancini, C., Ciaroni, S., Voci, A., Gerdoni, E. and Gallo, G., 2005. Thyroid hormones affect neurogenesis in the dentate gyrus of adult rat. Neuroendocrinology, 81(4), pp.244-253. PMID: 16113586
Chan, S. and Kilby, M.D., 2000. Thyroid hormone and central nervous system development. The Journal of Endocrinology, 165(1), p.1-8. PMID: 10750030
Constantinou, C., Margarity, M. and Valcana, T., 2005. Region-specific effects of hypothyroidism on the relative expression of thyroid hormone receptors in adult rat brain. Molecular and Cellular Biochemistry, 278(1-2), pp.93-100. PMID: 16180094
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