Anti-TFAM (Transcription Factor A, mitochondrial) Antibody

Name: Anti-TFAM (Transcription Factor A, mitochondrial) Antibody
Brand: PhosphoSolutions
SKU: 1999-hTFAM
Price: 394.0 USD
Availability: InStock

Our Anti-TFAM (Transcription Factor A, mitochondrial) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human TFAM (Transcription Factor A, motochondrial) and is neat serum. It is great for use in WB.

Western blot of HeLa lysate showing specific immunolabeling of the ~24 kDa TFAM protein.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

Trial Size

SKU: 1999-hTFAM

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

TFAM (Transcription Factor A, motochondrial)

Scientific Background

Mitochondrial Transcription Factor A (TFAM) is a key activator of mitochondrial (mt) DNA transcription as well as a participant in mitochondrial genome replication. mtDNA is highly susceptible to oxidative stress leading to mitochondrial dysfunction. Overexpression of TFAM has been implicated in the amelioration of age dependent impairment of brain functions through the prevention of oxidative stress and mitochondrial dysfunction in microglia (Hayashi et al., 2008). More recently, TFAM overexpression has been shown to potentially reduce oxidative stress in motor neurons and delay onset of amyotrophic lateral sclerosis (ALS) in ALS model mice (Morimoto et al., 2012).

Format

Neat Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

TFAM

Molecular Weight

24 kDa

Immunogen

Native recombinant human TFAM protein with c-terminal 6-his tag.

Immunogen Species

Human

Species Reactivity

Human

Antibody Registry ID

AB_2571641

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Neat Serum

Buffer

Neat Serum

Dilution Ranges

WB: 1:2000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~24 kDa TFAM protein. The antibody has been directly tested for reactivity in human. Shows lesser reactivity to rat and mouse due to some sequence homology, but our catalog # 2001-TFAM is recommended for use in rodent.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Transcription factor 6-like 1; Mitochondrial transcription factor 1; mitochondrial transcription factor; mitochondrial transcription factor A; MtTF1; mtTFA; TCF 6; TCF-6; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; TFAM_HUMAN; Transcription factor 6; Transcription factor 6 like 2 (mitochondrial transcription factor; Transcription factor 6 like 2; Transcription factor 6-like 2; transcription factor 6-like 3; Transcription factor A mitochondrial; Transcription factor A mitochondrial precursor; Transcription factor 6 like 2

UniProt ID

Q00059
UniProt Summary: Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed:19304746, PubMed:22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171). {ECO:0000269|PubMed:1737790, ECO:0000269|PubMed:19304746, ECO:0000269|PubMed:20410300, ECO:0000269|PubMed:22037171, ECO:0000269|PubMed:22037172, ECO:0000269|PubMed:22841477, ECO:0000269|PubMed:29149603, ECO:0000269|PubMed:29445193, ECO:0000269|PubMed:32183942}.

NCBI Gene ID

7019

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Hayashi, Y., Yoshida, M., Yamato, M., Ide, T., Wu, Z., Ochi-Shindou, M., Kanki, T., Kang, D., Sunagawa, K., Tsutsui, H. and Nakanishi, H., 2008. Reverse of age-dependent memory impairment and mitochondrial DNA damage in microglia by an overexpression of human mitochondrial transcription factor a in mice. Journal of Neuroscience, 28(34), pp.8624-8634. PMID: 18716221
Morimoto, N., Miyazaki, K., Kurata, T., Ikeda, Y., Matsuura, T., Kang, D., Ide, T. and Abe, K., 2012. Effect of mitochondrial transcription factor a overexpression on motor neurons in amyotrophic lateral sclerosis model mice. Journal of Neuroscience Research, 90(6), pp.1200-1208. PMID: 22354563

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Anti-TFAM (Transcription Factor A, mitochondrial) Antibody

SKU: 1999-hTFAM

Product Details

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