Our Anti-Synapsin I (Ser603) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Synapsin I (Ser603) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 603 is the site on synapsin I that is phosphorylated by calcium calmodulin kinase II and by p21-activated kinases (Sakurada et al., 2002; Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function (Nayak et al., 1996; Bahler and Greengard, 1987; McGuinness et al., 1989).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
SYN1
78 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser603 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Xenopus, Zebrafish
AB_2492247
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser603 . Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser603
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody
Magupalli, V.G., et al. 2013. Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2.1 calcium channels. Journal of Biological Chemistry, 288(7), 4637-4648.
Nitta, A., et al. 2021. Schizophrenia-like behavioral impairments in mice with suppressed expression of piccolo in the medial prefrontal cortex. Journal of Personalized Medicine, 11(7), p.607.
Takahashi, E., et al. 2010. Interaction Between CaV2.1 α 1 and CaMKII in CaV2.1 α 1 Mutant Mice, Rolling Nagoya. Journal of Molecular Neuroscience, 41(2), pp.223-229.
