Anti-Synapsin I (Ser603) Antibody

Name: Anti-Synapsin I (Ser603) Antibody
Brand: PhosphoSolutions
SKU: p1560-603
Price: 456.0 USD
Availability: InStock

Our Anti-Synapsin I (Ser603) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Synapsin I (Ser603) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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Species Reactivity

Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p1560-603

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Synapsin I (Ser603)

Scientific Background

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 603 is the site on synapsin I that is phosphorylated by calcium calmodulin kinase II and by p21-activated kinases (Sakurada et al., 2002; Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function (Nayak et al., 1996; Bahler and Greengard, 1987; McGuinness et al., 1989).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

SYN1

Molecular Weight

78 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser603 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Xenopus, Zebrafish

Antibody Registry ID

AB_2492247

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser603 . Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser603

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Brain protein 4.1; SYN 1; SYN 1a; SYN 1b; SYN I; SYN1; SYN1_HUMAN; SYN1a; SYN1b; Synapsin 1; Synapsin I; Synapsin-1; Synapsin1; SynapsinI; SYNI

UniProt ID

P17599
UniProt Summary: Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed:21441247, PubMed:23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity). {ECO:0000250|UniProtKB:O88935, ECO:0000250|UniProtKB:P09951, ECO:0000269|PubMed:21441247, ECO:0000269|PubMed:23406870}.

NCBI Gene ID

281510

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Blue Ice

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Anti-Synapsin I (Ser603) Antibody

SKU: p1560-603

Product Details

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