Our Anti-Synapsin I rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Synapsin I and is neat pooled serum. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.
Synapsin I plays a key role in synaptic plasticity in the brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).
Neat Pooled Serum
Polyclonal
IgG
WB
Rabbit
SYN1
78 kDa
Native protein purified from bovine brain.
Human, Mouse, Rat
AB_2492243
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Unpurified pooled neat serum
Neat serum
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~78 kDa synapsin I doublet. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody
Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. Journal of Neuroscience 22:4372- 4380. PMID: 12040043
Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ** (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. Journal of Neuroscience 21:7944-7953. PMID: 11588168
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ**, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neuroscience 5:431-437. PMID: 11976703
Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity. Journal of Neuroscience 9:2151-2162. PMID: 2498469
Nayak AS, Moore CI, Browning MD** (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. PNAS (USA) 93:15451-15456. PMID: 8986832
Stone LM, Browning MD**, Finger TE (1994) Differential distribution of the synapsins in the rat olfactory bulb. Journal of Neuroscience 14:301-309. PMID: 8283238
**Dr. Michael Browning and Dr. Andrew Czernik, co-authors of the cited papers are two of PhosphoSolutions' founders.
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