Immunoblot versus extracts of heart tissue from WT and SUR2 KO mice probed with N323A/31 TC supe. Data courtesy of William Coetzee and Margaret Rice, New York University SOM.
Anti-SUR1 and SUR2B Antibody
ATP-binding cassette sub-family C member 8 (Sulfonylurea receptor 1) ATP-binding cassette sub-family C member 9 (Sulfonylurea receptor 2)
SUR1 and SUR2B are members of the ATP binding cassette transporter subfamily C. These proteins combine with KCNJ8(Kir6.1) or KCNJ11(Kir6.2) to form K+ATP channels and are involved in their regulation and activation.
Abcc8 Sur Sur1 Abcc9 Sur2
175 kDa (and smaller fragments likely due to proteolytic cleavage)
Fusion protein amino acids 1503-1545 (VHTILTADLVIVMKRGNILEYDTPESLLAQEDGVFASFVRADM, cytoplasmic C-terminus) of rat SUR2B (also known as Sulfonylurea receptor 2B, ATP-binding cassette transporter subfamily C member 9B and Abcc9B, accession number Q63563-2); Mouse: 100% identity (43/43 amino acids identical); Human: 97% identity (42/43 amino acids identical); 75% identity with SUR1
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.