Anti-Superoxide dismutase 1 (SOD1) Antibody

Name: Anti-Superoxide dismutase 1 (SOD1) Antibody
Brand: Biosensis
SKU: R-168-100
Price: 337.0 USD
Availability: InStock

Our Anti-Superoxide dismutase 1 (SOD1) rabbit polyclonal primary antibody detects human Superoxide dismutase 1 (SOD1), and is whole serum. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

Immunohistochemical detection (DAB staining) of superoxide dismutase (SOD1) in paraffin embedded human brain tissue using Rabbit antibody to superoxide dismutase (SOD1; 121-139): whole serum (R-168-100) at a dilution of 1: 100 incubated overnight. Secondary antibody was biotinylated goat anti-rabbit used at a dilution of 1: 3000 together with Vector ABC. This antibody labels neurons and some glial cells in human brain tissue. Other tissues have not yet been tested.

Detection of SOD1 protein (16 kDa) in SH-SY5Y cell lysates with R-168-100. This antibody does not detect SOD1 in mouse and rat tissue samples. Proteins were separated by reduced SDS-PAGE, transferred to nitrocellulose membrane. Blots were incubated with R-168-100 at 1:1000 dilution using an anti-rabbit-HRP conjugate (1:10000) as secondary antibody.

Immunohistochemical detection (DAB staining) of superoxide dismutase (SOD1) in paraffin embedded human brain tissue using Rabbit antibody to superoxide dismutase (SOD1; 121-139): whole serum (R-168-100) at a dilution of 1: 100 incubated overnight. Secondary antibody was biotinylated goat anti-rabbit used at a dilution of 1: 3000 together with Vector ABC. This antibody labels neurons and some glial cells in human brain tissue. Other tissues have not yet been tested.

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Species Reactivity

Human

Applications

ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-168-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Product Details

Target

Superoxide dismutase 1 (SOD1)

Scientific Background

SOD1 binds copper and zinc ions ans is one of two isozymes responsible for destroying free superoxide radicals which are normally produced within the cells and which are toxic to biological systems. SOD1 is a soluble cytoplasmic protein, acting as a homodimer to convert superoxide radicals to molecular oxygen and hydrogen peroxide. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) which is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ICC, IHC, WB

Host Species

Rabbit

Immunogen

A peptide (HEKADDLGKGGNEESTKTG) corresponding to the amino acids 121-139 of human superoxide dismutase (SOD1) conjugated to diphtheria toxin has been used as the immunogen.

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

Physical State

Lyophilized

Dilution Ranges

WB: 1:1000-1:4000
IHC: 1:100-1:1000

Conjugate

Unconjugated

Specificity

This antibody has been shown to be specific for superoxide dismutase (SOD1) protein. Human. Does not detect mouse and rat SOD1.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Superoxide dismutase; SOD1;

UniProt ID

P00441

Shipping

25°C (ambient)

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Anti-Superoxide dismutase 1 (SOD1) Antibody

SKU: R-168-100

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