Anti-Stat5 (Tyr-694), Phosphospecific Antibody (M148)

Name: Anti-Stat5 (Tyr-694), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: SM1481
Price: 360.0 USD
Availability: InStock

Our Stat5 (Tyr-694) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Stat5 (Tyr-694) and is Protein A purified. It is great for use in WB, ICC.

Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 (SM2511) or Stat5 (Tyr-694) (SM1481) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: SM1481

Volume: 100 µL

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Price:

$360.00

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Product Details

Target

Stat5 (Tyr-694)

Target Description

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFNα in B cells, but only Stat5A is phosphorylated in response to IFNα in HeLa cells.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M148

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

STAT5A

Molecular Weight

Antigen

Clone (M148) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 694 of human Stat5A. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 92 kDa* band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells treated with EGF, but does not detect this band in control cells.

Post Translational Modification Type

GOmAb/Phosphorylated

Post Translational Modification Residue

Tyr-694

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Signal transducer and activator of transcription 5A, Stat5A

UniProt Details

UniProt (Human): P42229
UniProt (Immunogen Species): P42229

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Anti-Stat5 (Tyr-694), Phosphospecific Antibody (M148)

SKU: SM1481

Product Details

Bulk Order Anti-Stat5 (Tyr-694), Phosphospecific Antibody

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