Anti-Stat3 (N-terminal region) Antibody (M263)

Name: Anti-Stat3 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SM2631
Price: 275.0 USD
Availability: InStock

1 Citation

Our Stat3 (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Stat3 (N-terminal region) and is Protein A purified. It is great for use in WB, IP.

Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).

Species Reactivity

Human, Mouse, Rat

Applications

IP, WB

Host Species

Mouse

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SKU: SM2631

Volume: 100 µL

Variant

Price:

$275.00

Quantity:

Product Details

Target

Stat3 (N-terminal region)

Target Description

The stat proteins (Stat1-6) function both as cytoplasmic signal transducers and as activators of transcription in response to cytokines and growth factor receptors. Stat3 is expressed as two variants, Stat3α (86 kDa) and Stat3b (79 kDa) that can differ in expression and activity depending on cell type, activation pathway, and cell maturation stage. Both are activated by phosphorylation at Tyr-705, which induces dimerization, nuclear translocation and DNA binding. Stat3α (86 kDa) transcriptional activation may be regulated by phosphorylation at Ser-727 through the MAPK pathway, while Stat3β lacks this serine site.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M263

Isotype

IgG1

Applications

IP, WB

Host Species

Mouse

Gene Name

STAT3

Molecular Weight

79/86

Antigen

Clone (M263) was generated from a recombinant protein that included amino acid residues in the N-terminal region of human Stat3. This sequence has high homology to the conserved regions in rat and mouse Stat3 α and β.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects 86 and 79 kDa* bands corresponding to Stat3 isoforms on SDS-PAGE immunoblots of human A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Signal transducer and activator of transcription 3

UniProt Details

UniProt (Human): P40763
UniProt (Immunogen Species): P40763

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
30941654	not listed	Hu, C, et al. 2019. Resveratrol inhibits the proliferation of estrogen receptor-positive breast cancer cells by suppressing EZH2 through the modulation of ERK1/2 signaling. Cell biology and toxicology, 445-456.

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Anti-Stat3 (N-terminal region) Antibody (M263)

SKU: SM2631

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Stat3 (N-terminal region) Antibody

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