Our Anti-Stargazin rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Stargazin and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat synaptic plasma membrane (SPM) lysate showing specific immunolabeling of the ~36 kDa stargazin protein.
Stargazin is a member of the transmembrane AMPAR regulatory proteins (TARP) family and is involved in glutamate receptor trafficking. It has been recently demonstrated (Tomita et al., 2005; Priel et al., 2005) that the interaction between stargazin and AMPA receptors is critical for the correct localization of the receptors at the synapse. Phosphorylation of the stargazin protein at Thr-321- by Protein Kinase A regulates its interaction with PSD-95 and synaptic targeting of AMPA receptors (Choi et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
CACNG2
36 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat Stargazin, conjugated to keyhole limpet hemocyanin (KLH).
Rat
Mouse
AB_2492240
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification using a column to which the C-terminal peptide imunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~36kDa stargazin protein. Immunolabeling of the stargazin band is blocked by preadsorption with the peptide used as antigen.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Selvakumar, B., et al. 2014. AMPA receptor upregulation in the nucleus accumbens shell of cocaine-sensitized rats depends upon S-nitrosylation of stargazin. Neuropharmacology, 77, 28-38.
