Anti-Spectrin alpha chain, non-erythrocytic 1 Antibody (3D7)

Our Anti-Spectrin alpha chain, non-erythrocytic 1 mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Spectrin alpha chain, non-erythrocytic 1, and is affinity-purified. It is validated for use in FC, ICC, IHC-Frozen, WB.

Left: Confocal image of mixed neuron-glial cultures stained with Mouse monoclonal antibody to non-erythrocyte alpha-spectrin M-1575-100 (green) and counterstained for DNA (blue). M-1575-100 stains numerous axonal and dendritic profiles in these cultures, and this image shows an optical section through a group of neuronal cell bodies. M-1575-100 clearly reveals the submembraneous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody. This antibody also reveals the submembranous cytoskeleton of the axon. Right: Analysis of non-erythrocyte alpha spectrin expression in human neuroblastoma SH-SY5Y cell line by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to Non-erythrocyte alpha spectrin (cat # M-1575-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Left: Rat cerebellum stained for alpha II spectrin (green, 1:2,000) and GFAP (C-1373-50, red, 1:5,000). The spectrin antibody stains the submembraneous cytoskeleton on neurons and strongly reveals the cell bodies and dendrites of Purkinje cells, while the GFAP antibody stains the processes of Bergmann glia and astrocytes. Right: Western blot analysis of spectrin expression (green) in neural tissue and cell lysates. [1] protein standard, [2] rat whole brain, [3] rat spinal cord, [4] mouse whole brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, [10] C6 glioma cells. A prominent band at about 250-260 kDa represents the intact alpha II spectrin heavy chain.
Left: Confocal image of mixed neuron-glial cultures stained with Mouse monoclonal antibody to non-erythrocyte alpha-spectrin M-1575-100 (green) and counterstained for DNA (blue). M-1575-100 stains numerous axonal and dendritic profiles in these cultures, and this image shows an optical section through a group of neuronal cell bodies. M-1575-100 clearly reveals the submembraneous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody. This antibody also reveals the submembranous cytoskeleton of the axon. Right: Analysis of non-erythrocyte alpha spectrin expression in human neuroblastoma SH-SY5Y cell line by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to Non-erythrocyte alpha spectrin (cat # M-1575-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Left: Confocal image of mixed neuron-glial cultures stained with Mouse monoclonal antibody to non-erythrocyte alpha-spectrin M-1575-100 (green) and counterstained for DNA (blue). M-1575-100 stains numerous axonal and dendritic profiles in these cultures, and this image shows an optical section through a group of neuronal cell bodies. M-1575-100 clearly reveals the submembraneous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody. This antibody also reveals the submembranous cytoskeleton of the axon. Right: Analysis of non-erythrocyte alpha spectrin expression in human neuroblastoma SH-SY5Y cell line by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to Non-erythrocyte alpha spectrin (cat # M-1575-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
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Bovine, Human, Mouse, Pig, Rat
Flow, ICC, IHC, WB
Mouse

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Data Sheet

SKU: M-1575-100

Volume/Size: 100 µg
Price:
$327.00

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