Anti-SOX2 Antibody

Name: Anti-SOX2 Antibody
Brand: Aves Labs
SKU: SOX2-0100
Price: 481.0 USD
Availability: InStock

Our anti-SOX2 chicken primary antibody from Aves Labs is manufactured in-house. It detects SOX2, and is affinity purified. It is provided in liquid form.

Immunofluorescent staining of NTERA2 cells stained with Aves Labs anti-SOX2 antibody (green) showing strong nuclear staining of endogenous SOX2. Actin filaments are stained with phalloidin (red).

Western blotting of SOX2-FLAG transfected COS-7 cell lysate (10 µg/lane), mock transfected COS-7 cell lysate (10 µg/lane) and NTERA2 cell lysate (10 µg/lane) and stained with Aves Labs anti-SOX2 antibody (1µg/mL).Note that SOX2 runs at higher molecular weight in lane 1 due to presence of tandem Myc/FLAG tag on recombinant protein relative to endogenous SOX2 in lane 3.

Transverse section of an E11.75 mouse embryonic face. SOX2 (Cat. SOX2, 1:100; red) staining seen in nasal epithelium and neuroepithelium. Nuclei stained with DAPI (blue). Image kindly provided by Viviana Hermosilla, University of California, San Francisco.

Immunofluorescent staining of NTERA2 cells stained with Aves Labs anti-SOX2 antibody (green) showing strong nuclear staining of endogenous SOX2. Actin filaments are stained with phalloidin (red).

Click on image to zoom

Applications

ICC, WB

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Data Sheet

Trial Size

SKU: SOX2-0100

Volume: 500 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Format

Affinity-Purified IgY

Concentration

0.2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, WB

Gene Name

SOX2

Molecular Weight

35 kDa

Immunogen

His tagged full length protein.

Immunogen Species

Human

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:500-1:1000
ICC: 1:100-1:500

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Synonyms

ANOP3; Sex Determining Region Y-Box 2; SRY (sex determining region Y-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2; SRY-Box Transcription Factor 2; SRY-Box 2; MCOPS3

UniProt ID

P48431
UniProt Summary: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development.

NCBI Gene ID

6657

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Anti-SOX2 Antibody

SKU: SOX2-0100

Product Details

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