Our Anti-SNAP25 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat SNAP25 and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific labeling of the ~25 kDa SNAP25 protein.
SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer’s Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, WB
Rabbit
SNAP25
25 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat SNAP25, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification using a column to which the C-terminal peptide imunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~25 kDa SNAP25 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
bA416N4.2; Bdr; CMS18; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC 9; SEC9; SNAP 25; SNAP; SNAP-25; SNAP-25B; SNAP25; SNP 25; SNP25; SNP25_HUMAN; sp; SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal associated protein 25; Synaptosomal associated protein 25kDa; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; Synaptosomal-associated protein 25-KD
P60881
UniProt Summary: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. {ECO:0000250|UniProtKB:P60881}.
Fatemi, S.H., Earle, J.A., Stary, J.M., Lee, S. and Sedgewick, J., 2001. Altered levels of the synaptosomal associated protein SNAP-25 in hippocampus of subjects with mood disorders and schizophrenia. Neuroreport, 12(15), pp.3257-3262. PMID: 11711867
Fatemi, S.H., Sidwell, R., Kist, D., Akhter, P., Meltzer, H.Y., Bailey, K., Thuras, P. and Sedgwick, J., 1998. Differential expression of synaptosome-associated protein 25 kDa [SNAP-25] in hippocampi of neonatal mice following exposure to human influenza virus in utero. Brain Research, 800(1), pp.1-9. PMID: 9685568
Greber, S., Lubec, G., Cairns, N. and Fountoulakis, M., 1999. Decreased levels of synaptosomal associated protein 25 in the brain of patients with Down syndrome and Alzheimer's disease. ELECTROPHORESIS: An International Journal, 20(4‐5), pp.928-934. PMID: 10344268
Ando, K., Kudo, Y. and Takahashi, M., 2005. Negative regulation of neurotransmitter release by calpain: a possible involvement of specific SNAP‐25 cleavage. Journal of Neurochemistry, 94(3), pp.651-658. PMID: 15992386
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