Anti-Small ubiquitin-related modifier 1 (SUMO-1) Antibody
Our Anti-Small ubiquitin-related modifier 1 (SUMO-1) sheep polyclonal primary antibody detects human and rat Small ubiquitin-related modifier 1 (SUMO-1), and is whole serum. It is validated for use in IHC-Frozen, WB.

Western blot detection of recombinant SUMO-1 protein using Sheep antibody to SUMO-1 (6-21): ws, (catalogue no. S-064-100) at a dilution of 1:8000. The protein sample was 1 µl of E.Coli cell lysate containing approximately 50 ng of recombinant SUMO-1 protein. This antibody recognises a major band of approximately 17 kDa corresponding to SUMO-1, as well as a smaller minor band of 8 kDa.
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SKU: S-064-100
Product Details
Small ubiquitin-related modifier 1 (SUMO-1)
SUMO-1 binds to a wide range of target proteins as part of a post-translational modification system. Unlike ubiquitin, it does not seem to target protein for degradation, but is involved in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis, apoptosis, protein stability and signal transduction. SUBUNIT: Covalently attached to a number of proteins such as PmL, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain. PTM: Cleavage of the last four amino acids of the carboxy-terminus of the precursor form by SENP1 or SENP2 is necessary for function. Several pseudogenes have been reported as well as a number of alternatively spliced isoforms.
Whole serum
Polyclonal
Mixed
IHC, WB
Sheep
A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Human
Human, Rat
Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Lyophilized
WB: 1:4000-1:8000
IHC: 1:2000-1:4000
IHC: 1:2000-1:4000
Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used.
Unconjugated
This antiserum recognizes human SUMO-1 and not ubiquitin. This antiserum is known to cross react with rat and human SUMO-1.
For research use only.
United States
12 months after date of receipt (unopened vial).
Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
25°C (ambient)