Anti-SHP1 (Tyr-536), Phosphospecific Antibody

Name: Anti-SHP1 (Tyr-536), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: SP1571
Price: 329.0 USD
Availability: InStock

16 Citations

Our SHP1 (Tyr-536) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat SHP1 (Tyr-536) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to alkaline phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Tyr-536) antibody (lanes 3-5). The SHP1 (Tyr-536) antibody was used in the presence of phospho-SHP1 (Tyr-536) peptide (lane 5).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Go to Full Product Details

SKU: SP1571

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

SHP1 (Tyr-536)

Target Description

SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKCα and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

PTPN6

Molecular Weight

Antigen

Phospho-SHP1 (Tyr-536) synthetic peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 536 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide then affinity-purified using phospho-SHP1 (Tyr-536) peptide. The antibody detects a 68 kDa* band on SDS-PAGE immunoblots of human Jurkat cells treated with pervanadate, but is not observed in control cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-536

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PTP1C, SHPTP1

UniProt Details

UniProt (Human): P29350
UniProt (Immunogen Species): P29350

Product Specific References for Applications and Species

Flow Cytometry: Human
Immunocytochemistry: Human | Mouse
RPPA: Human
Western Blot: Human | Mouse | Rat

Flow Cytometry: Human
PMID	Dilution	Publication
24405902	not listed	Le Page, A, et al. 2014. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell communication and signaling, 2.

Immunocytochemistry: Human
PMID	Dilution	Publication
29307735	not listed	Le Page, A, et al. 2018. Signal transduction changes in CD4+ and CD8+ T cell subpopulations with aging. Experimental Gerontology, 128-139.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
25876760	not listed	Cerny, O, et al. 2015. Bordetella pertussis Adenylate Cyclase Toxin Blocks Induction of Bactericidal Nitric Oxide in Macrophages through cAMP-Dependent Activation of the SHP-1 Phosphatase. Journal of immunology (Baltimore, Md. : 1950), 4901-4913.

Reverse-Phase Protein Assay: Human
PMID	Dilution	Publication
29187853	not listed	Presotto, D, et al. 2017. Fine-Tuning of Optimal TCR Signaling in Tumor-Redirected CD8 T Cells by Distinct TCR Affinity-Mediated Mechanisms. Frontiers in immunology, 1564.

Western Blot: Human
PMID	Dilution	Publication
24405902	not listed	Le Page, A, et al. 2014. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell communication and signaling, 2.
24713856	not listed	Kataoka, TR, et al. 2013. CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 2861.
23391724	not listed	Hebeisen, M, et al. 2013. SHP-1 phosphatase activity counteracts increased T cell receptor affinity. The Journal of Clinical Investigation, 1044-1056.

Western Blot: Mouse
PMID	Dilution	Publication
28811476	1:1000	Mkaddem, SB, et al. 2017. Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation. Nature communications, 246.
28250424	not listed	Choi, S, et al. 2017. THEMIS enhances TCR signaling and enables positive selection by selective inhibition of the phosphatase SHP-1. Nature immunology, 433-441.
27271569	not listed	Moogk, D, et al. 2016. Constitutive Lck Activity Drives Sensitivity Differences between CD8+ Memory T Cell Subsets. Journal of immunology (Baltimore, Md. : 1950), 644-654.
25239131	not listed	Kataoka, TR, et al. 2015. CD72 negatively regulates mouse mast cell functions and down-regulates the expression of KIT and FcεRIα. International immunology, 95-103.
24990897	not listed	Zhou, X, et al. 2014. Leishmania infantum-chagasi activates SHP-1 and reduces NFAT5/TonEBP activity in the mouse kidney inner medulla. American Journal of Physiology. Renal Physiology, F516-524.
19201855	not listed	Verhagen, AM, et al. 2009. A kinase-dead allele of Lyn attenuates autoimmune disease normally associated with Lyn deficiency. Journal of immunology (Baltimore, Md. : 1950), 2020-2029.

Western Blot: Rat
PMID	Dilution	Publication
28017654	not listed	Park, SY, et al. 2017. Hippocalcin Promotes Neuronal Differentiation and Inhibits Astrocytic Differentiation in Neural Stem Cells. Stem Cell Reports, 95-111.
26342897	not listed	Choi, YR, et al. 2015. FcγRIIB mediates the inhibitory effect of aggregated α-synuclein on microglial phagocytosis. Neurobiology of Disease, 90-99.
24140598	not listed	Tibaldi, E, et al. 2014. The tyrosine phosphatase SHP-1 inhibits proliferation of activated hepatic stellate cells by impairing PDGF receptor signaling. Biochimica et Biophysica acta, 288-298.

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Anti-SHP1 (Tyr-536), Phosphospecific Antibody

SKU: SP1571

Product Details

Product Specific References for Applications and Species

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