Anti-ShcA Antibody

Name: Anti-ShcA Antibody
Brand: PhosphoSolutions
SKU: SP1331
Price: 275.0 USD
Availability: InStock

Our ShcA primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, rabbit, and rat ShcA and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of ShcA in paraformaldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal (top) and rabbit polyclonal (bottom) ShcA antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of ShcA expression in A431 cell lysate (lanes 1, 2, 3, & 4). The blots were probed with rabbit polyclonal anti-ShcA (SP1331) at 1:1000 (lane 1) or 1:4000 (lane 2) and mouse monoclonal ShcA (C-terminal region) at 1:1000 (lane 3) or 1:4000 (lane 4).

Species Reactivity

Human, Mouse, Rabbit, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: SP1331

Volume: 100 µL

Variant

Price:

$275.00

Quantity:

Product Details

Target

ShcA

Target Description

The adapter protein Shc was initially identified as an SH2 containing proto-oncogene involved in growth factor signaling. Since then, a number of studies in multiple systems have implicated a role for Shc in many different types of signal transduction including growth factor, antigen, cytokine, G-protein, hormone, and integrin receptor signaling. In addition to the ubiquitous ShcA, there are two other shc gene products, ShcB and ShcC, which are predominantly expressed in neuronal cells. ShcA knockout mice are embryonic lethal and have clearly suggested an important role for ShcA in vivo. An important role for Shc in the activation of MAPK pathway has been established. Thus, Shc adapter proteins are critical components of signal transduction pathways involved in many different cellular processes.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

SHC1

Molecular Weight

45/52/66

Antigen

A recombinant protein that included amino acid residues within the C-terminal region of human ShcA. This sequence has high homology with similar regions in rat and mouse ShcA.

Species Reactivity

Human, Mouse, Rabbit, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects 46/52/66 kDa* bands corresponding to the molecular weight of ShcA variants on SDS-PAGE immunoblots of A431 cells. It also detects ShcA in human Jurkat, HeLa, and SKN-SH cells, as well as rat brain tissue and rabbit spleen fibroblasts.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SH2 C1, SHC

UniProt Details

UniProt (Human): P29353
UniProt (Immunogen Species): P29353

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Anti-ShcA Antibody

SKU: SP1331

Product Details

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