Anti-Shank3 Antibody (N69/46)

Name: Anti-Shank3 Antibody
Brand: NeuroMab
SKU: 75-109
Price: 329.0 USD
Availability: InStock

Our Anti-Shank3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N69/46. It detects human, mouse, and rat Shank3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2, Shank3 or Kv2.1 plasmids and probed with N69/46 TC supe (left panel) or a monoclonal lane loading control (right panel).

Adult rat hippocampus immunohistochemistry.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-109

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Shank3

Target Description

SH3 and multiple ankyrin repeat domains protein 3, or shank 3 (also known as proline-rich synapse-associated protein 2 (ProSAP2)) is a member of the shank family. Shank 3 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Shank 3 is found in many tissues including heart and spleen, but is most abundantly expressed in brain at the post synaptic density. Shank 3 acts as a scaffold protein anchoring and connecting membrane bound receptors, ion channels and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 3 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 3 gene have been associated with neuropsychiatric diseases, including autism spectrm disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N69/46

Isotype

IgG2b

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Shank3 Prosap2

Molecular Weight

190 kDa

Antigen

Synthetic peptide amino acids 840-857 (PEKLPGSLRKGIPRTKSV) of rat Shank3 (accession number Q9JLU4)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2187730

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.37

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity against Shank1 or Shank2

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

UniProt ID

Q9JLU4

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Human | Mouse
Immuno Gold: Rat
Quantitative Multiplex Co-Immunoprecipitation: Mouse
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
24089484	1:500	Duffney LJ, et al. 2013. Shank3 deficiency induces NMDA receptor hypofunction via an actin-dependent mechanism.. Journal of Neuroscience, 15767-15778.

Immunocytochemistry: Rat
PMID	Dilution	Publication
21606927	not listed	Durand CM, et al. 2012. SHANK3 mutations identified in autism lead to modification of dendritic spine morphology via an actin-dependent mechanism.. Molecular Psychiatry, 71-84.

Immunohistochemistry: Human
PMID	Dilution	Publication
20573181	1:1000	Pham E, et al. 2010. Progressive accumulation of amyloid-beta oligomers in Alzheimer's disease and in amyloid precursor protein transgenic mice is accompanied by selective alterations in synaptic scaffold proteins. FEBS Journal, 3051-3067.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
25637745	not listed	Braude JP, et al. 2015. Deletion of Shank1 has minimal effects on the molecular composition and function of glutamatergic afferent postsynapses in the mouse inner ear.. Hearing Research, 52-64.
20573181	1:1000	Pham E, et al. 2010. Progressive accumulation of amyloid-beta oligomers in Alzheimer's disease and in amyloid precursor protein transgenic mice is accompanied by selective alterations in synaptic scaffold proteins. FEBS Journal, 3051-3067.

Immuno-Gold: Rat
PMID	Dilution	Publication
26215919	1:10	Farley MM, et al. 2015. Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities.. Neuroscience, 286-301.

Quantitative Multiplex Co-Immunoprecipitation: Mouse
PMID	Dilution	Publication
34852231	not listed	Jonathan D Lautz, et al. 2021. Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states. Cell Reports, 110076.

Western Blot: Human
PMID	Dilution	Publication
24089484	1:500	Duffney LJ, et al. 2013. Shank3 deficiency induces NMDA receptor hypofunction via an actin-dependent mechanism.. Journal of Neuroscience, 15767-15778.
20573181	1:1000	Pham E, et al. 2010. Progressive accumulation of amyloid-beta oligomers in Alzheimer's disease and in amyloid precursor protein transgenic mice is accompanied by selective alterations in synaptic scaffold proteins. FEBS Journal, 3051-3067.

Western Blot: Mouse
PMID	Dilution	Publication
38484863	1:1000	Kelly-Castro, et al. 2024. MARK1 regulates dendritic spine morphogenesis and cognitive functions in vivo. Experimental Neurology, 114752.
28934977	1:1000	Chhibber A, et al. 2017. ERβ and ApoE isoforms interact to regulate BDNF-5-HT2A signaling and synaptic function in the female brain. Alzheimers Research Therapy, 79.
26027926	not listed	Duffney LJ, et al. 2015. Autism-like Deficits in Shank3-Deficient Mice Are Rescued by Targeting Actin Regulators. Cell Reports, 1400-1413.
25997006	not listed	Benthani F, et al. 2015. Proteogenomic Analysis Identifies a Novel Human SHANK3 Isoform.. International Journal of Molecular Sciences, 11522-11530.
21167025	not listed	Bozdagi O, et al. 2010. Haploinsufficiency of the autism-associated Shank3 gene leads to deficits in synaptic function, social interaction, and social communication.. Molecular Autism, 15.
20573181	1:1000	Pham E, et al. 2010. Progressive accumulation of amyloid-beta oligomers in Alzheimer's disease and in amyloid precursor protein transgenic mice is accompanied by selective alterations in synaptic scaffold proteins. FEBS Journal, 3051-3067.

Western Blot: Rat
PMID	Dilution	Publication
28139198	1 : 100	Harony-Nicolas H, et al. 2017. Oxytocin improves behavioral and electrophysiological deficits in a novel Shank3-deficient rat.. Elife, e18904.
21606927	not listed	Durand CM, et al. 2012. SHANK3 mutations identified in autism lead to modification of dendritic spine morphology via an actin-dependent mechanism.. Molecular Psychiatry, 71-84.

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Anti-Shank3 Antibody (N69/46)

SKU: 75-109

Product Details

Product Specific References for Applications and Species

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