Anti-RBPMS Recombinant Guinea Pig Chimeric mAb (OTI3B7-GP)

Name: Anti-RBPMS Recombinant Guinea Pig Chimeric mAb
Brand: PhosphoSolutions
SKU: 1834-RBPMS
Price: 470.0 USD
Availability: InStock

This guinea pig RBPMS recombinant monoclonal antibody takes the place of our 1832-RBPMS guinea pig polyclonal antibody. It is a chimeric antibody, combining the antigen-binding variable region of mouse clone OTI3B7 with the constant region of the guinea pig IgG molecule, making it perfect for reproducible multiplexing. It detects human, mouse, and rat RBPMS and works in WB, IHC, ICC, and flow cytometry.

Representative section of mouse retina showing cells stained by anti-RBPMS antibody. The sections were
stained with PhosphoSolutions anti-RBPMS recombinant guinea pig monoclonal antibody (visualized in green) (Cat No. 1834-RBPMS) at 1:1000 dilution. Anti-RBPMS specifically stains the retinal ganglion cells in the mouse retina. Image courtesy of Angela Wong, UCLA.

Western blotting of rat heart lysate with anti-RBPMS recombinant guinea pig monoclonal antibody (Cat No. 1834-RBPMS) at 1:4000 dilution (0.25 μg/mL) and detected with anti-guinea pig-HRP. It recognizes endogenous RBPMS in the rat heart lysate at ~24 kDa.

Representative section of mouse retina showing cells stained by anti-RBPMS antibody. The sections were stained with PhosphoSolutions anti-RBPMS recombinant guinea pig monoclonal antibody (visualized in green) (Cat No. 1834-RBPMS) at 1:1000 dilution. Anti-RBPMS specifically stains the retinal ganglion cells in the mouse retina. Image courtesy of Angela Wong, UCLA.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

Flow, ICC, IHC, WB

Host Species

Guinea Pig

Conjugates and Formulations

490

550

594

650

Carrier-Free

Go to Full Product Details

Data Sheet

Trial Size

SKU: 1834-RBPMS

Volume: 100 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

RBPMS

Scientific Background

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014).

Format

Purified by Protein A Chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

OTI3B7-GP

Isotype

IgG

Applications

Flow, ICC, IHC, WB

Host Species

Guinea Pig

Gene Name

RBPMS

Molecular Weight

24 kDa

Immunogen

Full length human recombinant protein of human RBPMS (NP_001008712) produced in E.coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone OTI3B7 with guinea pig IgG heavy and light constant regions. As such this antibody retains the same binding performance as the original clone OTI3B7 but can be detected using standard anti-guinea pig secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000-1:5000
IHC: 1:250-1:2500
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~24 kDa RBPMS protein.

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat heart lysate and confirmed to stain the expected molecular weight band.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

24 months from date of receipt

Synonyms

FLJ32971 antibody, Heart and RRM expressed sequence antibody, Hermes antibody, RBP MS antibody, RBP-MS antibody, Rbpms antibody, RBPMS_HUMAN antibody, RNA binding protein gene with multiple splicing antibody, RNA-binding protein with multiple splicing antibody

UniProt ID

Q9WVB0

NCBI Gene ID

19663

Shipping

Shipped on ice packs

Bulk Order Anti-RBPMS Recombinant Guinea Pig Chimeric mAb

Customer Reviews

Write a review

Recover password

Create my account

Anti-RBPMS Recombinant Guinea Pig Chimeric mAb (OTI3B7-GP)

SKU: 1834-RBPMS

Product Details

Bulk Order Anti-RBPMS Recombinant Guinea Pig Chimeric mAb

Customer Reviews

Related Products

Recently Viewed