Anti-SARM1 Antibody (6174-11)

Name: Anti-SARM1 Antibody
Brand: Antibodies Incorporated
SKU: 77-522
Price: 319.0 USD
Availability: InStock

Our Anti-SARM1 mouse monoclonal primary antibody from Antibodies Incorporated is produced in-house from hybridoma clone 1561353. It detects human, mouse, and rat SARM1, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunofluorescent staining of COS-7 cells expressing SARM1-flag using Antibodies Incorporated mouse anti-SARM1 clone 6174-11 (green) and rabbit anti-flag (red). DAPI nuclear stain (blue) showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between mouse anti-SARM1 signal and anti-flag in transfected cells.

Left pane - Western blotting of rat, human, or mouse brain homogenate (20 ug/lane) and transfected COS7 cell lysates (5 ug/ml) with Antibodies Incorporated mouse anti-SARM1 antibody (clone 6174-11). Overexpressed SARM1 in lane 4 runs higher due to dual MYC-FLAG tag. Right pane- Western blotting of same lysates with Antibodies Incorporated anti-GRP75 antibody (clone N52A/42) as loading control.

Sagittal section of formalin-fixed, paraffin-embedded rat brain showing strong staining for SARM1 in the Purkinje layer of the cerebellum as expected. Inset image shows higher magnification. Sections were stained with Antibodies Incorporated mouse anti-SARM1 (clone 6174-11) antibody at 1:500 dilution and detected with anti-mouse HRP.

Immunofluorescent staining of COS-7 cells expressing SARM1-flag using Antibodies Incorporated mouse anti-SARM1 clone 6174-11 (green) and rabbit anti-flag (red). DAPI nuclear stain (blue) showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between mouse anti-SARM1 signal and anti-flag in transfected cells.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

Trial Size

SKU: 77-522

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

SARM1

Target Description

SARM1 ( Sterile Alpha and TIR Motif-Containing 1) is predominantly expressed in the nervous system, particularly in neurons, and is highly conserved across different species. It encodes a protein that contains several functional domains, including a sterile alpha motif (SAM) domain, a Toll/interleukin-1 receptor (TIR) domain, and a catalytic domain with NAD^+ hydrolase activity. SARM1 activation triggers a cascade of molecular events that leads to the breakdown of axonal structures and eventual degeneration. In response to injury, SARM1 is activated and catalyzes cleavage of NAD+ into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide. In the nervous system, NAD+ depletion is a critical step in the axonal degeneration process, known as Wallerian degeneration, and is essential for clearing damaged axons to allow for regeneration and repair. Inhibition of SARM1 or the preservation of NAD+ levels has been shown to protect axons from degeneration in experimental models, suggesting SARM1 as a potential therapeutic target for neurodegenerative diseases and nerve injuries. Research on SARM1 has primarily focused on its role in axonal degeneration and neuroprotection. Understanding the molecular mechanisms underlying SARM1 activation and its downstream effects may have implications for developing treatments for various neurological disorders, such as peripheral neuropathies, traumatic brain injury, and neurodegenerative diseases like Parkinson's and Alzheimer's.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

6174-11

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

SARM1

Molecular Weight

73 kDa

Antigen

Fusion protein amino acids 51-724 of human SARM1 (accession number Q6SZW1) produced recombinantly in E. Coli.

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2938811

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:500
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

UniProt Details

UniProt (Human): Q6SZW1
UniProt (Immunogen Species): Q6SZW1

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Anti-SARM1 Antibody (6174-11)

SKU: 77-522

Product Details

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