Our Anti-SARM1 Antibody primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat SARM1 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Immunofluorescence of transfected COS-7 cells specifically labeling the positive SARM1 cells using our anti-SARM1 antibody (1:50, red) and anti-FLAG antibody (green). The yellow color results from the overlap of cells labeled with both antibodies. DNA is labeled with DAPI (blue).
NAD(+) hydrolase sterile alpha and TIR motif-containing protein 1 (SARM1), is the fifth member of the Toll/Il-1 Receptor (TIR) domain protein family. The highly conserved 724 amino acid protein is predominantly expressed in the brain, kidney and liver (Mink, M., et al 2001). Furthermore, SARM1 is enriched in the cytoplasm, mitchondria, and the axons, dendrites, and synapses of neurons (Panneerselvam, P., et al, 2012). SARM1 plays a key role in axonal degeneration by catalyzing the cleavage of NAD+ into ADPR, cADPR, and nicotinamide in response to injury triggering Wallerian degeneration (Gerdts, J. et al 2015). SARM1 has also been shown to activate neuronal cell death in response to stress (Peng, J. et al. 2010).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, WB
Rabbit
SARM1
79 kDa
Full length recombinant human SARM1 protein.
Human, Mouse, Rat
AB_2922825
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column to which the recombinant protein antigen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
ICC: 1:50
Unconjugated
Specific for endogenous levels of the ~79 kDa SARM1 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
FLJ36296 antibody, KIAA0524 antibody, MyD88-5 antibody, SAM and ARM-containing protein antibody, SAM domain-containing protein 2 antibody, SAMD2 antibody, SARM 1 antibody, SARM1 antibody, SARM1_HUMAN antibody, Sterile alpha and Armadillo repeat protein antibody, sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila antibody, sterile alpha and HEAT/Armadillo motifs-containing protein antibody, sterile alpha and TIR motif containing 1 antibody, Sterile alpha and TIR motif-containing protein 1 antibody, Sterile alpha and TIR motifs-containing protein 1 antibody, Sterile alpha motif domain-containing protein 2 antibody, Tir-1 homolog antibody
