Anti-S-arrestin (S-AG) Antibody (S128)
Our Anti-S-arrestin (S-AG) mouse monoclonal primary antibody detects bovine and pig S-arrestin (S-AG), and is affinity-purified. It is validated for use in IHC-Frozen, WB.

Left: Immunohistochemical analysis of arrestin-1 distribution in pig retina. Arrestin (green) is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL) and can be used to identify components of rod photoreceptor cells (cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The RNA binding protein FOX2 appears red and immunoreactivity is seen in nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA appears blue. Right: Western blot analysis of arrestin in bovine retinal extracts. The antibody stains one specific band at 48 kDa, corresponding to retinal arrestin.
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SKU: M-1796-100
Product Details
S-arrestin (S-AG)
Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (PubMed: 8003967, PubMed: 25205354). May play a role in preventing light-dependent degeneration of retinal photoreceptor cells (By similarity). Ref: uniprot.org
Affinity-purified
Monoclonal
S128
IgG1
IHC, WB
Mouse
Recombinant bovine arrestin-1 with the first 20 amino acids of the C-terminus truncated
Bovine
Bovine, Pig
Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Lyophilized
Protein G purified
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide.
WB: 1:5000
IHC: 1:1000
IHC: 1:1000
Unconjugated
Bovine, reacts with pig. Other species not yet tested. Antibody detects arrestin-1 protein only and does not cross-react with the other 3 arrestin molecules.
For research use only.
United States
12 months after date of receipt (unopened vial).
48 kDa protein, Retinal S-antigen, S-AG, Rod photoreceptor arrestin
25°C (ambient)