Anti-Robo1 (C-terminal region) Antibody

Name: Anti-Robo1 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: RP2791
Price: 292.0 USD
Availability: InStock

Our Robo1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Robo1 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of HepG2 (lanes 1 & 4), C2C12 (lanes 2 & 5), and HUVEC (lanes 3 & 6). The blot was probed with anti-Robo1 (C-terminal region) in absence (lanes 1-3) or presence of Robo1 (C-terminal region) blocking peptide (RX2795; lanes 4-6).

Immunocytochemical labeling of Robo1 in mouse C2C12. The cells were labeled with rabbit polyclonal Robo1 (C-terminal region) in the absence or presence of blocking peptide (RX2795). The antibody was then detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of HepG2 (lanes 1 & 4), C2C12 (lanes 2 & 5), and HUVEC (lanes 3 & 6). The blot was probed with anti-Robo1 (C-terminal region) in absence (lanes 1-3) or presence of Robo1 (C-terminal region) blocking peptide (RX2795; lanes 4-6).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: RP2791

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Robo1 (C-terminal region)

Scientific Background

The Robo family of repulsive guidance receptors (Robo1-4) have important roles in controlling axon guidance and cell migration. These receptors are members of the immunoglobulin (Ig) superfamily and consist of an ectodomain with five Ig domains and three fibronectin type III repeats, a single transmembrane domain, and a long cytoplasmic tail that contains four blocks of conserved cytoplasmic sequences. In Drosophila, mutations in Robo, and its midline-expressed ligand Slit, result in too many axons crossing and staying at the midline. Several proteins that regulate the actin cytoskeleton, including cAbl, Ena, and Rho-family GTPases, contribute to the Robo signaling pathway. cAbl phosphorylates Robo1 at Tyr-1073, and this may inhibit Robo activity, while Slit-Robo signaling activates both Rac and Rho, and inactivates Cdc42. Thus, Robo guidance receptors control axon outgrowth and cell migration through activation of cell signaling pathways that regulate cytoskeletal dynamics.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, WB

Host Species

Rabbit

Gene Name

ROBO1

Molecular Weight

180

Immunogen

Robo1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human Robo1. This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family members.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a 180 kDa* band corresponding to Robo1 in Western blots of human HepG2 and HUVEC cells, as well as mouse C2C12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

DUTT1; roundabout homolog 1; deleted in U twenty twenty; roundabout; axon guidance receptor; homolog 1

UniProt ID

Q9Y6N7
UniProt Summary: Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.

NCBI Gene ID

6091

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Anti-Robo1 (C-terminal region) Antibody

SKU: RP2791

Product Details

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