Our Anti-Ribosomal S6 kinase 2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Ribosomal S6 kinase 2 and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~90 kDa RSK2 protein.
The p90 ribosomal S6 kinases (RSK)1–4 are downstream members of the extracellular signal-regulated kinase (ERK)/MAPK cascade. The loss of RSK2 activity in humans leads to Coffin–Lowry syndrome, which is characterized by mental retardation and growth deficits (Hanauer and Young, 2002). Recent work suggests that RSK2 exerts a tonic regulation on G-protein coupled signaling (Sheffler et al., 2006).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
RPS6KA2
90 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region on mouse ribosomal S6 kinase 2, conjugated to keyhole limpet hemocyanin (KLH).
Human
Bovine, Canine, Chicken, Mouse, Rat
AB_2492233
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column to which C-terminal peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~90 kDa RSK2 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Hegde, S., et al. 2016. Phosphodiesterase 11A (PDE11A), enriched in ventral hippocampus neurons, is required for consolidation of social but not nonsocial memories in mice. Neuropsychopharmacology, 41(12), p. 2920-2931.
