Anti-Ribosomal protein S6 (Ser244) Antibody

Name: Anti-Ribosomal protein S6 (Ser244) Antibody
Brand: PhosphoSolutions
SKU: p1860-244
Price: 456.0 USD
Availability: InStock

Our Anti-Ribosomal protein S6 (Ser244) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse Ribosomal protein S6 (Ser244) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of Jurkat cell lysate showing specific immunolabeling of the ~28 kDa rpS6 phosphorylated at Ser244 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

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Species Reactivity

Human, Mouse

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p1860-244

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ribosomal protein S6 (Ser244)

Scientific Background

Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–Akt–mTOR pathway (Chen and Dittmer 2011).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

RPS6

Molecular Weight

28 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 of mouse rpS6, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Mouse

Species Reactivity

Human, Mouse

Expected Reactivity

Chicken, Goat, Non-Human Primate, Rat, Xenopus, Zebrafish

Antibody Registry ID

AB_2492234

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~28 kDa rpS6 protein phosphorylated at Ser244. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser244

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

40S ribosomal protein S6; Air8; NP33; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; rps6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein

UniProt ID

P62754
UniProt Summary: Component of the 40S small ribosomal subunit (PubMed:23636399, PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797). {ECO:0000269|PubMed:17220279, ECO:0000269|PubMed:23636399, ECO:0000269|PubMed:34516797, ECO:0000269|PubMed:8706699}.

NCBI Gene ID

20104

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Chen, W. and Dittmer, D.P., 2011. Ribosomal protein S6 interacts with the latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus. Journal of Virology, 85(18), pp.9495-9505. PMID: 21734034
Fumagalli, S. and Thomas, G., 2000. 23-S6 Phosphorylation and Signal Transduction. Cold Spring Harbor Monograph Archive
, 39, pp.695-717.Hutchinson, J.A., Shanware, N.P., Chang, H. and Tibbetts, R.S., 2011. Regulation of ribosomal protein S6 phosphorylation by casein kinase 1 and protein phosphatase 1. Journal of Biological Chemistry, 286(10), pp.8688-8696. PMID: 21233202

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Anti-Ribosomal protein S6 (Ser244) Antibody

SKU: p1860-244

Product Details

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