Anti-RGS14 Recombinant Chicken Chimeric mAb FL594 Conjugate (N133/21)

Name: Anti-RGS14 Recombinant Chicken Chimeric mAb FL594 Conjugate
Brand: Aves Labs
SKU: 78-170-FL594
Price: 562.0 USD
Availability: InStock

Our chicken Anti-RGS14 FL594 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N133/21. It detects mouse and rat RGS14 and is purified by affinity chromatography. KO validated. Works in IHC, ICC.

Anti-RGS14 Recombinant Chicken Chimeric mAb FL594 Conjugate

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Applications

ICC, IHC

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 78-170-FL594

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

RGS14

Scientific Background

RGS14 is a member of the RGS family (regulator of G-protein signaling) and is highly expressed in the prefrontal cortex. Hallmark domains of RGS14 include a RGS domain, two Raf-like Ras-binding domains and a GoLoco motif. RGS14 inactivates G protein α subunits. Alterations in this protein have been implicated in Schizophrenia and Alzheimer’s disease.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

N133/21

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

Rgs14

Molecular Weight

60 kDa

Excitation / Emission

FL594 Ex: 594 nm, Em: 615 nm

Immunogen

Fusion protein amino acids 1-544 (full-length) of rat RGS14 (accession number O08773) produced recombinantly in E. Coli

Immunogen Species

Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N133/21 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N133/21 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

594

Specificity

Does not react with RGS12

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

regulator of G-protein signaling 14; RGS14

UniProt ID

O08773
UniProt Summary: Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Besides, modulates signal transduction via G protein alpha subunits by functioning as a GDP-dissociation inhibitor (GDI). Has GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not on GNAI2 and G(o)-alpha subunit GNAO1. Has GAP activity on GNAI0, GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance. May be involved in visual memory processing capacity and hippocampal-based learning and memory.

NCBI Gene ID

114705

Shipping

Shipped on ice packs

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Anti-RGS14 Recombinant Chicken Chimeric mAb FL594 Conjugate (N133/21)

SKU: 78-170-FL594

Product Details

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