Our Anti-REDD1 (Thr23/25) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human REDD1 (Thr23/25) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of jurkat cell lysate showing specific immunolabeling of the ~34 kDa REDD1 protein phosphorylated at Thr23/25 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).
REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli (Schwarzer et al, 2005). REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress (Katiyar et al, 2009). It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A–DDB1–ROC1–b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr-25, Thr-23 and Ser-19 through the activity of GSK3b (Katiyar et al, 2009).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
DDIT4
34 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 of human REDD1, conjugated to keyhole limpet hemocyanin (KLH).
Human
Non-Human Primate
AB_2492227
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:500
Unconjugated
Specific for endogenous levels of the ~34 kDa REDD1 phosphorylated at Thr23/25. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Thr23,25
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Schwarzer, R., Tondera, D., Arnold, W., Giese, K., Klippel, A. and Kaufmann, J., 2005. REDD1 integrates hypoxia-mediated survival signaling downstream of phosphatidylinositol 3-kinase. Oncogene, 24(7), p.1138. PMID: 15592522
Katiyar, S., Liu, E., Knutzen, C.A., Lang, E.S., Lombardo, C.R., Sankar, S., Toth, J.I., Petroski, M.D., Ronai, Z.E. and Chiang, G.G., 2009. REDD1, an inhibitor of mTOR signalling, is regulated by the CUL4A–DDB1 ubiquitin ligase. EMBO Reports, 10(8), pp.866-872. PMID: 19557001
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